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Skupne fenolne spojine in antioksidativna učinkovitost ekstrakta kimchija
Hribar, Kaja (Author), Pogačnik, Lea (Mentor) More about this mentor... This link opens in a new window, Terpinc, Petra (Reviewer)

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Abstract
Kimchi je tradicionalna fermentirana jed, ki izvira iz Koreje. Namen diplomskega dela je bilo pripraviti domači kimchi in ga primerjatis kupljenim. Tako iz kupljenega kot domačega kimchija smo želeli pripraviti vzorce soka in ekstrakte odcejenega kimchija ter v njih določiti skupne fenolne spojine in antioksidativno učinkovitost (AOU). V raziskavi smo uporabili pakirani korejski kimchi baechu znamke Chongga in kimchi, ki smo ga pripravili sami, in sicer s sedemdnevno in tridnevno fermentacijo ter sveži nefermentirani kimchi. V pripravljenih vzorcih soka in ekstrakta kimchija smo pred opravljanjem analiz določili vrednost pH z uporabo pH lističev. Vsebnost skupnih fenolnih spojin smo v vzorcih domačega in kupljenega kimchija določili spektrofotometrično z uporabo Folin‒Ciocalteujevega reagenta in rezultate analize izrazili kot ekvivalent klorogenske kisline. AOU soka in ekstrakta kimchija smo določili z metodo lovljenja radikala DPPH• in rezultate analize podali kot ekvivalent troloksa. Najvišjo AOU smo določili v vzorcu domačega kimchija, ki smo ga pustili fermentirati tri dni (0,22 mg/g v soku in 0,38 mg/g v ekstraktu), in v vzorcu kupljenega kimchija (0,24 mg/g v vzorcu soka in 0,44 mg/g v ekstraktu). Ta dva vzorca sta se tudi najbolj približala v literaturi opisani optimalni vrednosti pH 4,2. Povprečna vrednost skupnih fenolnih spojin v vzorcih soka domačega kimchija znaša 0,27 mg/g, v ekstraktu pa 0,33 mg/g. V soku kupljenega kimchija je vsebnost 0,53 mg/g, v ekstraktu pa 0,42 mg/g. Iz opravljene raziskave lahko sklepamo, da čas fermentacije in vrednost pH, ki jo živilo doseže, vplivata na vsebnost fenolnih spojin in AOU.

Language:Slovenian
Keywords:fermentirana živila, kimchi, sok iz kimchija, ekstrakt iz kimchija, fenolne spojine, antioksidanti, antioksidativna učinkovitost, analizne metode, Folin‒Ciocalteu, DPPH
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2017
Publisher:[K. Hribar]
UDC:663/664:547.56:543.2
COBISS.SI-ID:4830584 Link is opened in a new window
Views:775
Downloads:416
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Secondary language

Language:English
Title:Analysis of total phenolic compounds and antioxidant activity in kimchi extracts
Abstract:
Kimchi is a traditional Korean fermented vegetable containing several ingredients. The purpose of this thesis was to compare homemade nonfermented fresh kimchi as well as kimchi fermented for seven and three days with store-bought kimchi by determining antioxidant activity (AOU) and the total phenolic content in kimchi juice and extracts. In our research, we used beachu kimchi by brand Chongga as well as kimchi made at home from locally bought ingredients. We measured the pH value of our samples with the use of pH paper. Total phenolic content of extracts was determined spectrophotometrically according to Folin‒Ciocalteu method, the results of the analysis were expressed as an equivalent of the chlorogenic acid. AOU was evaluated using DPPH• radical scavenging method. AOU was expressed as trolox equivalent. The highest AOU was determined in homemade kimchi that was left to ferment for three days (0,22 mg/g in juice and 0,38 mg/g in extract) as well as in the store-bought kimchi (0,24 mg/g in juice and 0,44 mg/g in extract). The pH values of these samples were close to the optimum pH value reported elsewhere (pH 4,2). The average total phenolic compounds in homemade kimchi juice was 0,27 mg/g and in in extracts 0,33 mg/g. The total phenolic compounds in store-bought kimchi juice was 0,53 mg/g and in extract 0,42 mg/g. From our research it can be concluded that fermentation duration and the pH value of kimchi affects the total phenolic content and antioxidant activity.

Keywords:fermented foods, kimchi, kimchi juice, kimchi extracts, total phenolic compounds, antioxidants, antioxidant activity, analytic methods, Folin-Ciocalteu, DPPH

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