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Vpliv živega srebra in selena na biomolekularne profile v prehranjevalni verigi.
Gregorič, Petra (Author), Vogel Mikuš, Katarina (Mentor) More about this mentor... This link opens in a new window, Grdadolnik, Jože (Co-mentor)

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Abstract
Živo srebro (Hg) je za organizme strupeno že pri nizkih koncentracijah. Raziskave kažejo, da selen (Se) lahko zmanjšuje negativne učinke Hg tako pri rastlinah kot živalih. Namen naloge je bil razviti metodologijo uporabe biomolekularnih profilov (BMP) rastlin in živali izmerjenih z metodo infrardeče spektroskopije s Fourjerjevo transformacijo (FTIR) v kombinaciji z ustreznimi statističnimi metodami, kot potencialnih biooznačevalcev izpostavljenosti Hg in/ali Se. Kot testna organizma smo izbrali solato in polža lazarja. Pri solati smo zabeležili spremembe v BMP zaradi izpostavljenosti Hg že pri odmerku 1 mg Hg/kg suhih tal dodanega v obliki HgCl2. Tako v koreninah kot poganjkih se je statistično najznačilneje povečal signal vibracijskih trakov (VT) v območjih 1599-1615 cm-1 in 1369-1425 cm-1, ki sta značilni za proteine in pa statistično najznačilneje zmanjšal signal VT v območjih 1010-1036 cm-1, 1039-1077 cm-1 (poganjki), 990-988 cm-1 (korenine) in 832-939 cm-1 (korenine), ki so značilni za ogljikove hidrate. V koreninah je Se pri nižjih odmerkih Hg omilil omenjene spremembe, pri višjih odmerkih Hg pa ni imel več vpliva. V poganjkih je Se omilil spremembe pri izpostavitvi odmerku 100 mg Hg/kg suhih tal, pri manjših odmerkih pa ni imel vpliva. Spremembe ob izpostavitvi Hg se kažejo tudi v lipidnem delu FTIR spektra. Tudi pri polžih, ki so uživali solato s povečanimi vsebnostmi Hg, se kažejo spremembe BMP. V prebavni žlezi in mišičnem tkivu se statistično najznačilneje zmanjšajo intenzitete VT v območju 1630-1649 cm-1 (proteini). Statistično najznačilneje pa se povečajo v območju 1387-1415 cm-1 (prebavna žleza, proteini), 1451-1465 cm-1 in 1402-1407 cm-1 (mišica, proteini). V lipidnem delu so vidne spremembe predvsem v prebavni žlezi. Selen je omenjene spremembe omilil.

Language:Slovenian
Keywords:Živo srebro, selen, prehranjevalna veriga, solata, polž, FTIR, biokemijske spremembe
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2017
Publisher:[P. Gregorič]
UDC:543.422.3.74:582.991.41:594.3:57.088.6:546.23:543.272.81(043.2)
COBISS.SI-ID:8762745 Link is opened in a new window
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Downloads:378
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Secondary language

Language:English
Title:Influence of mercury and selenium on the biomolecular profiles in food chain
Abstract:
Mercury (Hg) is toxic for organisms, even at low concentrations. Studies have shown that selenium (Se) may reduce the negative effects of the Hg in plants and animals. The purpose of the thesis was to develop a methodology for the use of biomolecular profiles (BMP) of plants and animals as potential biomarkers of Hg and/or Se exposure. We measured the selected test organisms (lettuce and snails) by the method of Fourier transform infrared spectroscopy (FTIR) with the appropriate statistical approaches. In the case of lettuce we observed changes in the BMP when exposed to Hg at a dose of 1 mg Hg/kg dry soil, added in the form of HgCl2. In the roots and shoots there was a statistically significant increase of signal vibratory strip (VS) in the regions 1599-1615 cm-1 and 1369-1425 cm-1, which are characteristic of proteins and decrease of signal VS in the areas 1010-1036 cm-1, 1039-1077 cm-1 (shoots), 990-988 cm-1 (roots), and 832-939 cm-1 (roots), which are specific for carbohydrates. At lower doses of Hg, Se mitigated changes in roots, but at higher doses of Hg, Se did not have influence anymore. In the shoots it mitigated changes when exposed to a dose of 100 mg Hg/kg dry soil, but at lower doses had no effect on toxicity. Because of exposure to Hg, changes are also present in the lipid part of the FTIR spectrum. Even snails, which were feed with lettuce with elevated Hg contents, show changes of BMP. The statistically significant intensity of VS in the range of 1630-1649 cm-1 (proteins) is significantly reduced in samples of snails digestive gland and muscle tissue. The statistically significant increases of VS are present in the range of 1387-1415 cm-1 (digestive gland, proteins), 1451-1465 cm-1 and 1402-1407 cm-1 (muscle, proteins). In lipid part of spectrum we notice the changes mainly in the digestive gland when we added Se. It has mitigated these changes.

Keywords:Mercury, slenium, food chain, lettuce, snail, FTIR, biochemical changes

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