Uporaba biotehnoloških metod za izboljšanje lastnosti medicinske konoplje (Cannabis sativa L.).

Dorn, Romana (author), Bohanec, Borut (mentor), Flajšman, Marko (comentor)

Medicinska konoplja (Cannabis sativa L.) je rastlina, ki lahko zaradi vsebnosti kanabinoidov in drugih snovi, ki vplivajo na zdravje, marsikateremu bolniku izboljša življenje. Glavna kanabinoida, ki se nahajata v konoplji, sta delta-9-tetrahidrokanabinol (Δ9THC) in kanabidiol (CBD), ki se sintetizirata v žleznih trihomih. V človeku kanabinoidi delujejo preko endokanabinoidnega sistema. Δ9THC je psihoaktivna substanca, ki vpliva na povečanje apetita, zmanjšuje slabost, deluje proti bolečinam in stimulira imunski sistem, CBD pa poleg zdravilnih učinkov zmanjšuje neželene učinke Δ9THC. Zaradi vse večje uporabe konoplje v medicini je želja pridobiti rastline z različnimi vsebnostmi kanabinoidov in doseči čim večje donose za izolacijo. Uporaba biotehnoloških metod in razvoj novih protokolov za uspešno shranjevanje, razmnoževanje, gensko transformacijo in regeneracijo rastlin vodi v učinkovitejše žlahtnjenje medicinske konoplje.

• Language: Slovenian
• Keywords: biotehnološke metode, medicinska konoplja, Cannabis sativa, kanabinoidi, žlahtnjenje rastlin
• Work type: Final seminar paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [R. Dorn]
• Year: 2017
• PID: 20.500.12556/RUL-92922
• UDC: 633.522:602.6(043.2)
• COBISS.SI-ID: 8764793
• Publication date in RUL: 09.07.2017

Secondary language

• Language: English
• Title: Use of biotechnological methods for improving characteristics of medicinal cannabis (Cannabis sativa L.)

Abstract

Medical cannabis (Cannabis sativa L.) is a plant which can improve the quality of life for many patients because the content of cannabinoids and other substances that affect health. The main cannabinoids in cannabis are delta-9-tetrahydrocannabinol (Δ9THC) and cannabidiol (CBD), which are synthesized in glandular trihomes. Cannabinoids work through the endocannabinoid system in humans. Δ9THC is a psychoactive substance that affects appetite, reduces nausea, acts against pain and stimulates the immune system. CBD, in addition to the therapeutic effects, reduces adverse effects of Δ9THC. Due to the increasing use of cannabis in medicine, the goal is to acquire plants with different cannabinoid contents and to achieve the highest possible yields in cannabinoids extractions. The use of biotechnological methods and the development of new protocols for successful storage, micropropagation, gene transformation, plant regeneration lead to more efficient breeding of medical cannabis.

• Keywords: biotechnological methods, medicinal cannabis, Cannabis sativa, cannabinoids, plant breeding