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Mikropropagacija genskih virov evropskega pravega kostanja (Castanea sativa Mill) napadenih s kostanjevo šiškarico (Dryocosmus kuriphilus Yasumatsu).
Vollmeier, Rebecca (Author), Luthar, Zlata (Mentor) More about this mentor... This link opens in a new window

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Abstract
V Sloveniji je evropski pravi kostanj (Castanea sativa Mill.) zelo poškodovan od napada kostanjeve šiškarice (Dryocosmus kuriphilus Yasumatsu). V letih 2015 in 2016 smo v poskus in vitro razmnoževanja vključili sadjarsko zanimive genotipe iz različnih območij Slovenije z namenom pridobiti mlad, zdrav sadilni material. Izsečke za zasnovo in vitro kulture smo pridobili iz odgnalih brstov na poganjkih, ki so bili 10 – 14 dni v vodi. Nastale poganjke smo površinko razkužili z 1,66 % dikloroizocianurno kislino in iz njih pripravili nodijske izsečke, ki smo jih inokulirali na MS-½NO3 razmnoževalno gojišče. Spremljali smo okuženost kulture ter nastanek vitalnih, vitrificiranih in propadlih poganjkov. Ugotovili smo, da med genotipi obstajajo statistično značilne razlike v regeneraciji vitalnih, vitrificiranih in propadlih poganjkov. Genotipa, ki sta se od ostalih značilno razlikovala sta bila z oznako Š2 iz leta 2015 in Š4 iz leta 2016. Potrdili smo tudi, da ima leto vzorčenja značilen vpliv na regeneracijo vitalnih poganjkov, prav tako tudi interakcija genotip:leto, medtem ko interakcija genotip:subkultivacija nima značilnega vpliva. In vitro koreninjenje je bilo neuspešno, saj nismo uspeli pridobiti vitalnih poganjkov s koreninami. Ugotovili smo, da ima največji vpliv na uspešnost mikropropagacije genotip ter njegovo fiziološko stanje.

Language:Slovenian
Keywords:rastlinske tkivne kulture, evropski pravi kostanj, Castanea sativa Mill., kostanjeva šiškarica, Dryocosmus kuriphilus Yasumatsu, mikropropagacija, regeneracija, koreninjenje
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2017
Publisher:[R. Vollmeier]
UDC:634.53:57.086.83:631.532(043.2)
COBISS.SI-ID:8735353 This link opens in a new window
Views:1150
Downloads:615
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Secondary language

Language:English
Title:Micropropagation of the european chestnut genetic resources (Castanea sativa Mill.) attacked with chestnut gall wasp (Dryocosmus kuriphilus Yasumatsu)
Abstract:
In Slovenia, the European chestnut (Castanea sativa Mill.) is threatened to collapse due to the attack of the chestnut gall wasp (Dryocosmus kuriphilus Yasumatsu). In an experiment of vegetative propagation, between 2015 and 2016, we have included different interesting genotypes of chestnut from different regions of Slovenia that were damaged due to the attack. Our goal was to obtain healthy planting material. Explants for the design of in vitro culture were obtained from herded buds on the shoots, which we left 10 – 14 days in the water. The resulting shoots were surface-disinfected with 1.66 % dichloroisocyanuric acid. From disinfected shoots we prepared node explants which were then inoculated on an MS-½NO3 multiplication medium. We monitored the contamination of culture and the emergence of viable, vitrified and degraded shoots. We have found that between genotypes there exist statistically significant differences in the regeneration of viable, hyperhydric (vitrified) and degraded shoots. Genotypes which were statistically different from the remaining genotypes were genotypes Š2 from 2015 and Š4 from 2016. We confirmed that the year of sampling in the regeneration of vital shoots has impact on regeneration and that the interaction genotype:year is statistically significant, while the interaction genotype:subcultivation is not statistically significant. In vitro rooting was unsuccessful, because we were not able to obtain vital shoots with roots. We found that the greatest impact on micropropagation has genotype and its physiological condition.

Keywords:plant tissue cultures, european chestnut, Castanea sativa Mill., chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu, mikropropagation, regeneration, rooting

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