Introduction: Relation between falls, balance and walking are complex. For this reason, many balance tests as well as walk tests were developed – in order to predict the risks of falling. Purpose: The purpose of this final thesis is to determine differences between genders, between dominantly and non-dominantly extremity, between positions and repeatability of the medio-lateral step test. Methods: Research included 60 younger subjects who performed the test of stepping in the medio-lateral direction in a sitting and standing position. The data obtained were statistically analyzed. Results: In comparison with women, men were faster and have shown better results. ICC values for individual showed satisfactory reliability. Dominance of the leg has a significant impact on the execution of the test, especially in an upright position. Discussion and conclusion: Based on the results, we can speak about the reliability of the medio-lateral stepping test. However, the dominance of the extremity should be a subject of further research.