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Izbris gena za egerolizin v bakterijskem sevu Pseudomonas aeruginosa PA01
Tršan, Melisa (Author), Butala, Matej (Mentor) More about this mentor... This link opens in a new window

URLURL - Presentation file, Visit http://pefprints.pef.uni-lj.si/3905/ This link opens in a new window

Abstract
V diplomskem delu predstavim proteine iz družine egerolizinov in se osredotočim na protein RahU bakterije Pseudomonas aeruginosa. Bakterija P. aeruginosa je oportunistični patogen, ki izkorišča šibkost gostiteljevega imunskega sistema in povzroča številne okužbe. Predstavim pomen produkta gena pri obrambi bakterije. Osredotočim se na pripravo delecijske mutante bakterije P. aeruginosa. Deletirala sem gen rahU v genomu bakterije P. aeruginosa, tako da je v mutanti zamenjan z genom za odpornost proti antibiotiku kanamicinu. Zamenjavo genov sem izvedla s homologno rekombinacijo z rekombinaznim sistemom Lambda Red. Namen uporabe omenjenega molekularnega orodja je uspešna pridobitev delecijske mutante, ki nam v nadaljnjih analizah služi za prepoznavo funkcije proučevanega proteina z neznano funkcijo, naprimer proteina RahU.

Language:Slovenian
Keywords:egerolizini
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:PEF - Faculty of Education
Year:2016
COBISS.SI-ID:11224649 Link is opened in a new window
Views:551
Downloads:149
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Secondary language

Language:English
Title:Deletion of aegerolysin gene in strain of bacteria Pseudomonas aeruginosa PA01
Abstract:
The dissertation discusses the proteins from the aegerolysin family, specifically the RahU protein of the opportunistic pathogen bacteria, Pseudomonas aeruginosa. The biology of P. aeruginosa, which exploits its host’s weak immune system to cause numerous infections, is described. The main focus of the dissertation was the preparation of the deletion mutants. The rahU gene was deleted from the P. aeruginosa genome by replacing it with the kanymicin cassette. The gene replacement was carried out by using the Lambda Red recombinase system. The purpose of this molecular tool was to gain the rahU deletion mutants, which enable us to obtain new insights into the biology of the RahU protein.

Keywords:aegerolysins

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