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Rapid estimation of tocopherol content in linseed and sunflower oils-reactivity and assay
Prevc, Tjaša (Author), Levart, Alenka (Author), Kralj Cigić, Irena (Author), Salobir, Janez (Author), Poklar Ulrih, Nataša (Author), Cigić, Blaž (Author)

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Abstract
The reactivity of tocopherols with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was studied in model systems in order to establish a method for quantifying vitamin E in plant oils. The method was optimized with respect to solvent composition of the assay medium, which has a large influence on the course of reaction of tocopherols with DPPH. The rate of reaction of α-tocopherol with DPPH is higher than that of γ-tocopherol in both protic and aprotic solvents. In ethyl acetate, routinely applied for the analysis of antioxidant potential (AOP) of plant oils, reactions of tocopherols with DPPH are slower and concentration of tocopherols in the assay has a large influence on their molar reactivity. In 2-propanol, however, two electrons are exchanged for both α- and γ-tocopherols, independent of their concentration. 2-propanol is not toxic and is fully compatible with polypropylene labware. The chromatographically determined content of tocopherols and their molar reactivity in the DPPH assay reveal that only tocopherols contribute to the AOP of sunflower oil, whereas the contribution of tocopherols to the AOP of linseed oil is 75%. The DPPH assay in 2-propanol can be applied for rapid and cheap estimation of vitamin E content in plant oils where tocopherols are major antioxidants.

Language:English
Keywords:plant oil, α-tocopherol, γ-tocopherol, vitamin E, DPPH, antioxidants, solvent
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
FKKT - Faculty of Chemistry and Chemical Technology
Year:2015
Number of pages:Str. 14777-14790
Numbering:Vol. 20, iss. 8
UDC:664.3:577.161.3:544.4
ISSN on article:1420-3049
DOI:10.3390/molecules200814777 This link opens in a new window
COBISS.SI-ID:4555896 This link opens in a new window
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Downloads:339
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Record is a part of a journal

Title:Molecules
Shortened title:Molecules
Publisher:MDPI
ISSN:1420-3049
COBISS.SI-ID:18462981 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:13.08.2015

Secondary language

Language:Slovenian
Keywords:rastlinska olja, sončnično olje, laneno olje, antioksidanti, tokoferol, vitamin E, antioksidativni potencial, kemijska kinetika

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