Identification of types of ectomycorrhizae on seedlings in a beech provenance trial

Westergren, Marjana (author), Grebenc, Tine (author), Božič, Gregor (author), Brus, Robert (author), Kraigher, Hojka (author)

Root systems and types of ectomycorrhizae (ECM) were analysed on three 7-year-old beech seedlings (Fagus sylvatica L.) of three provenances (Val di Sella, Idrija, Nizbor) from an international beech provenance trial and on a naturally regenerated 5-year-old beech seedling from the same site (Kamenski vrh by Novo mesto). All short roots were mycorrhizal. In the sampled 20,302 root tips 49 % were non-turgescent and unidentifiable ECM. Twenty-two different types of ECM were identified, out of which 11 were successfully determined either to the species or genus level. All ECM were described by morphological and anatomical characteristics, basidiomycetes also by molecularmethods. ECM that was not successfully determined either to the species or genus level formed a new cluster in the Slovenian mycorrhizal molecular database. Species richness and percentage of vital ECM roots were highest for the provenance from Idrija (Slovenia). The results indicate that there might be differences between provenances regarding the abundance of ECM,which should be further studied.

• Language: English
• Keywords: beech, provenance trial, ectomycorrhizae, types of ectomycorrhizae
• Work type: Scientific work
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2004
• Number of pages: Str. 87-104
• Numbering: Št. 75
• PID: 20.500.12556/RUL-82970
• UDC: 630*114.7--01:232.41:176.1 Fagus sylvatica L.(045)
• ISSN on article: 0351-3114
• COBISS.SI-ID: 1437350
• Publication date in RUL: 18.08.2016

Record is a part of a journal

• Title: Zbornik gozdarstva in lesarstva. forest and wood science & technology
• Shortened title: Zb. gozd. lesar.
• Publisher: Gozdarski inštitut Slovenije, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
• ISSN: 0351-3114
• COBISS.SI-ID: 6206978

Secondary language

• Language: Slovenian
• Title: Identifikacija tipov ektomikorize na sadikah v bukovem provenienčnem poskusu

Abstract

V okviru mednarodnega bukovega provenienčnega poskusa na Kamenskem vrhu pri Novem mestu so bili v 3 ponovitvah analizirani koreninski sistemi in tipi ektomikorize (ECM) na 3 proveniencah (Val di Sella, Idrija, Nizbor) 7 letnih sadik bukve (Fagus sylvatica L.) in na 5 letnem vzorcu naravnega mladja. Skupno smo pregledali 20.302 korenini, od katerih je bilo 49 % starih, neturgescentnih ali nedoločljivih. Vse korenine so bile mikorizne. Ločili smo 22 različnih tipov ECM, od katerih smo 11 uspeli določiti do vrste ali rodu. Vse tipe smo opisali po morfološko-anatomskih kriterijih, bazidiomicete smo dodatno določevali z molekularnimi metodami. Neidentificirani tipi so tvorili v Slovenski mikorizni molekularni bazi podatkov nove grozde. Izračunani so bili indeksi raznovrstnosti. Največja diverziteta in delež vitalnih ektomikoriznih korenin sta bila ugotovljeni za provenienco iz Idrije. Pokazaleso se manjše razlike med proveniencami glede prisotnosti ektomikorize,kar zahteva nadaljnje raziskave.

• Keywords: bukev, Fagus sylvatica L., provenienčni poskus, ektomikoriza, tipi ektomikorize, PCR-RFLP