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Uporabniški vmesnik za avtonomno krmiljenje sistema za merjenje električnih lastnosti ravninskih lipidnih dvoslojev
KUMER, ROK (Author), Kramar, Peter (Mentor) More about this mentor... This link opens in a new window

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Abstract
Ravninski lipidni dvosloj lahko obravnavamo kot preprost model celične membrane in ga zaradi obojestranske električne dostopnosti uporabljamo pri meritvah električnih lastnosti celičnih membran. Poznavanje teh lastnosti je ključnega pomena za boljše razumevanje pojava elektroporacije na membrani. Za meritve električnih lastnosti celičnih membran potrebujemo veliko časa, saj je za vsako meritev potrebno ponovno zgraditi nov ravninski lipidni dvosloj, obenem pa potrebujemo veliko število podatkov za zagotovitev kvalitetnih in ponovljivih rezultatov. V ta namen smo v okolju MATLAB izdelali uporabniški vmesnik, ki omogoča enostaven vnos parametrov meritev ter minimalno prisotnost uporabnika. Uporabniški vmesnik med meritvami krmili platformo Red Pitaya, preko katere upravlja injekcijske črpalke za izgradnjo ravninskih lipidnih dvoslojev po metodi Montal – Mueller. S krmiljenjem signalnega generatorja Red Pitaye tvorimo napetostne ali tokovne signale in merimo napetostni odziv na ravninskem lipidnem dvosloju ali transmembranski tok, s katerima lahko izmerimo električne lastnosti ravninskih lipidnih dvoslojev. Naš cilj je bil s pomočjo Red Pitaye zajemati tudi odziv ravninskega lipidnega dvosloja ter obdelava tako pridobljenih podatkov, vendar pa v času naših poizkusov sočasno enkratno vzbujanje in zajemanje odziva na vzbujanje ravninskega lipidnega dvosloja še ni bilo mogoče, ker interna sinhronizacija v Red Pitayi še ni bila narejena.

Language:Slovenian
Keywords:avtonomno izvajanje meritev, uporabniški vmesnik, Matlab, ravninski lipidni dvosloj
Work type:Undergraduate thesis (m5)
Organization:FE - Faculty of Electrical Engineering
Year:2016
Views:790
Downloads:595
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Secondary language

Language:English
Abstract:
Planar lipid bilayer can be perceived as a simplified model of a cell membrane and can be used in measuring electrical properties of the said membrane, due to its electrical accessibility on both sides. Knowledge of these properties is of key importance for better understanding the phenomenon of cell membrane electroporation. While measuring electrical properties of a cell membrane is a time consuming process, due to having to rebuild the planar lipid bilayer for every measurement, a large number of measurements is required to provide accurate results. For this purpose an easy-to-use user interface has been designed in MATLAB, that simplifies measurement parameter entry and requires minimal attendance of its user. The user interface is used as a controller for the Red Pitaya platform which in turn controls the injection pumps used to construct a planar lipid bilayer using the Montal-Mueller technique. By controlling Red Pitaya signal generator voltage and current signals can be generated, which in turn enables measurements of voltage excitation response and transmembrane current, from which electrical properties of a planar lipid bilayer can be calculated. Another goal of this thesis was to measure the response of the planar lipid bilayer to the excitation using the Red Pitaya platform, which was not possible at the time tests were performed, due to Red Pitaya not being able to generate a one-time signal and acquiring it simultaneously.

Keywords:autonomous measuring, user interface, Matlab, planar lipid bilayer

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