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Genomska tipizacija izolatov virusa hepatitisa C v Sloveniji
Seme, Katja (Author), Poljak, Mario (Author), Čelan-Lucu, Branka (Author), Lešničar, Gorazd (Author), Brinovec, Vladimir (Author), Matičič, Mojca (Author), Meglič-Volkar, Jelka (Author), Žužek-Rešek, Saša (Author), Štepec, Srečko (Author), Baklan, Zvonko (Author), Andoljšek, Dušan (Author), Benedik-Dolničar, Majda (Author), Dovč, Peter (Author), Koren, Srečko (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:DOC-4XVPEZEJ This link opens in a new window

Abstract
Genotyping of hepatitis C virus (HCV) isolates is of particular interest for epidemiology. Because of extreme genome variability of HCV genome, each genotyping method should be evaluated in local setting before being introducedas a routine diagnostic procedure or a tool for large-scale epidemiological studies. This comparative study of four most widely used HCV genotyping assays, employed on 40 HCV isolates in Slovenia, showed that none of them was realy suitable for genotyping Slovenian HCV isolates. Thanks to its satisfactory sensitivity, specificity and simplicity, the line probe assaywas found to be the most appropriate tool for both routine and large-scale epidemiological studies. Using this assay the prevalence of HCV genotypes in Slovenia was studied on 226 individuals infected with HCV Significant differences in HCV genotype distribution observed between epidemiological groups in Slovenia indicate a close relationship between individual HCV genotypes in Slovenia was studied on 226 individuals infected with HCV. Significant differences in HCV genotype distribution observed between epidemiological groups in Slovenia indicate a close relationship between individual HCV gentoypes and certain routes of viral transmission.

Language:Slovenian
Keywords:epidemiologija, genotip, hepatitis, virusi, virusni hepatitis
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:1997
Publisher:Medicinski razgledi
Number of pages:Str. 345-362
Numbering:Letn. 36, št. 3
UDC:61
ISSN on article:0025-8121
COBISS.SI-ID:7593689 This link opens in a new window
Views:1010
Downloads:225
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Record is a part of a journal

Title:Medicinski razgledi
Shortened title:Med. razgl.
Publisher:Medicinski razgledi
ISSN:0025-8121
COBISS.SI-ID:3390978 This link opens in a new window

Secondary language

Language:English
Title:Genotyping of hepatitis C virus isolates in Slovenia
Abstract:
Opredelitev genotipa virusa hepatitisa C (HCV) je pomembna v raziskovanju epidemiologije okužbe s tem virusom oz. za natančno spremljanje ter razjasnitev načina prenosa in širjenja okužbe. Zaradi spremenljivosti genoma in neenakomerne zemljepisne porazdelitve genotipov HCV je treba pred začetkom kakrčnekoli epidemiološke raziskave v vsaki državi preveriti učinkovitost različnih tipizacijskih metod s pilotsko raziskavo in ob tem ugotoviti tudi okvirno prisotost in delež posameznih genotipov HCV S primerjalno raziskavo, ki smo jo izvedli na 40 izolatih RNA HCV iz Slovenije, smo primerjali učinkovitost štirih posrednih metod genomske tipizacije in ugotovili, da s posameznimi metodami ni mogoče dokončno opredeliti vseh genotipov oz. podtipovHCV, prisotnnih v Sloveniji. Izkazalo se je, da je v našem prostoru najbolj primerna metoda genomske tipizacije reverzni dot-blot s tipsko specifičnimi DNA-lovkami za 5'nekodirajoči del genoma HCV. Z izbrano strategijo genomske tipizacije smo nato določ/podtip HCV pri 226 slovenskih bolnikih, ki so se okužili s HCV na različne načine oz. so prpov HCV se v SLoveniji razlikuje med posamezninikov. Rezultati raziskave kažejo na povezavomed posamezdoločenimi načini prenosa virusa.


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