The main task was to determine a solvent system for the separation of fir lignans by thin layer chromatography. The dry substance of in fir samples was determined. We used Soxhlet method device firstly to extract hydrophobic (lipophilic) components with cyclohexane, and then to extract hydrophilic components with a mixture of water and acetone, the ratio of 95:5. Obtained from extracts, we determined the proportion of dry matter in the extract. We also carried out extraction with acetone without prior extraction with cyclohexane. The resulting sample extracts were then related to the TLC plate and developed in the mobile phase. Dichloromethane to ethanol volume ratio of 93:7 was used for the mobile phase, and H2SO4 / ethanol reagent volume ratio of 50:50 for spots visualization. The retention factor was determined from the spots on the chromatogram. Based on a comparison of our results with data from the literature, some organic substances which were in the extracts of knots were also determined.
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