Morphological and genetic variability of pedunculate oak (Quercus robur L.) populations in Slovenia

Batič, Franc (author), Sinkovič, Tomaž (author), Javornik, Branka (author)

The article presents a taxonomic study carried out in three pedunculate oak populations in Slovenia by leaf morphometry and by DNA analysis with RAPD markers. About one hundred leaves were sampled from five trees from three seminatural pedunculate oak stands in the Sub-pannonian vegetation region of Slovenia. Samples were collected from the well lit upper part of the tree crowns, and only leaves from short twigs were analysed. Leaf samples were herbarised, and measured and assessed in the laboratory. Leaf dimension (lamina length and width, petiole length, number of lobe pairs) were measured and characters like type of leaf pubescence and leaf lamina base type were assessed. Analyse of leaf morphometry showed that ali sampled trees belonged to the Quercus robur L. species. Nevertheless, on some trees, leaves also showed characters typical of sessile oak (Quercus petraea /Matt./ Leibl.), especially the leaf lamina base typ,3 and number of lobe pairs. Other parameters analysed were within the variation of pedunculate oak. More populations of both oak species should be analysed, especially when growing together, in order to obtain reliable data for taxonomic studies of oak hybrids and population genetic diversity by means of classic leaf and acorn morphometry and molecular markers.

• Language: English
• Keywords: pedunculate oak, morphology, multivariate analysis, RAPD markers, genetic polymorphism, natural hybridisation
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 1995
• Number of pages: Str. 75-96
• Numbering: Št. 46
• PID: 20.500.12556/RUL-69640
• UDC: 631.523.5:631.524.01:581.4:582Quercus robur(497.4)(045)
• ISSN on article: 0351-3114
• COBISS.SI-ID: 1987961
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Zbornik gozdarstva in lesarstva. forest and wood science & technology
• Shortened title: Zb. gozd. lesar.
• Publisher: Gozdarski inštitut Slovenije, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
• ISSN: 0351-3114
• COBISS.SI-ID: 6206978

Secondary language

• Language: Slovenian
• Title: Morfološka in genetska variabilnost populacij doba (Quercus robur L.) v Sloveniji

Abstract

Delo predstavlja taksonomske raziskave pri treh populacijah doba in vključuje morfometrijske meritve listov in DNK analizo z uporabo RAPD markerjev. Analizirali smo do 100 listov iz petih označenih dreves v subpanonskem vegetacijskem območju. Nabrali smo jih dobro osvetljene zgornje tretjine krošnje samo s kratkih poganjkov. Liste smo herbarizirali, merili in ovrednotili v laboratoriju. Ovrednoteni parametri listov so obsegali dolžino in širino listne ploskve, dolžino listnega peclja, število listnih krp, tip listnega dna in gostoto ter tip dlačic na listih. Raziskave morfometrijskih podatkov listov so pokazale, da raziskovana drevesa pripadajo dobu (Quercus robur L.). Posamezna drevesa na različnih ploskvah kažejo nekatere morfološke lastnosti gradna (Quercus petraea /Matt./Liebl.), posebej tip listnega dna in število listnih krp. Ostali morfološki parametri so v okviru variacijske širine doba. Analizirati bi morali večjo populacijo dreves predvsem v mešanih sestojih, kjer omenjeni vrsti rasteta skupaj. Podatki iz večje populacije bi bolj zanesljivo prikazali taksonomske probleme hibridov pri hrastih s primerjavo klasičnih morfoloških parametrov in molekularnih markerjev.

• Keywords: dob, quercus robur, genetska variabilnost, morfologija, RAPD, multivariatna analiza, naravna hibridizacija, Slovenija