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Primerjava imunoglobulina in paraproteina z nenavadno lastnostjo obarjanja : (magistrsko delo)
ID Jerković, Ksenija (Author), ID Glavač, Damjan (Mentor) More about this mentor... This link opens in a new window

URLURL - Presentation file, Visit https://dk.um.si/IzpisGradiva.php?id=47042 This link opens in a new window

Abstract
V kliničnih laboratorijih se pri analizi vzorcev srečujemo z različnimi motnjami meritev. V laboratoriju UKC Maribor smo ob mešanju seruma s kislo demineralizirano vodo naleteli na zanimiv pojav obarjanja serumskih proteinov in ga kmalu povezali z monoklonsko gamopatijo. Pojav monoklonskega imunoglobulina v serumu se kaže kot posledica skupine bolezenskih stanj. Nas je zanimalo, zakaj se proteini pri omenjenih pogojih oborijo le v nekaterih vzorcih z monoklonskim imunoglobulinom. Oborjene proteine smo poimenovali paraproteini z nenavadno lastnostjo obarjanja. Ker se proteini normalno ne obarjajo pri takih pogojih, so nas zanimali lastnosti in sestava paraproteina te vrste in vzrok za njegovo obarjanje. Raziskovalna metodologija V raziskavi smo izolirali paraprotein z nenavadno lastnostjo obarjanja in ga s kapilarno elektroforezo ločili na proteinske frakcije. Paraproteinu smo z dvodimenzionalno gelsko elektroforezo izdelali proteomski profil in določili molekulske mase fragmentov paraproteina. Z omenjenimi metodami smo poskušali identificirati paraprotein in izvedeti čim več o njegovih lastnostih. Teoretični del raziskave obsega statistično obdelavo biooznačevalcev vzorcev seruma z nenavadno lastnostjo obarjanja paraproteina in vzorcev z monoklonskim imunoglobulinom, pri katerih se paraprotein ne obori. Rezultati Ugotovili smo razlike med vzorci serumov z monoklonskim imunoglobulinom, pri katerih ne pride do obarjanja, in serumov, pri katerih se paraprotein obori. Rezultati kapilarne elektroforeze paraproteina so pokazali, da je večji delež paraproteina v obliki monoklonskega imunoglobulina. Imunski kompleksi na proteinogramih paraproteina niso bili vidni. Proteomski profili paraproteina kažejo prisotnost težkih in lahkih verig imunoglobulina. Masni spektri vzorcev paraproteinov so pokazali zmes več visokomolekularnih proteinov, vendar večjim molekulam zaradi omejitev metode nismo mogli določiti molekulske mase.

Language:Slovenian
Keywords:paraprotein, obarjanje, monoklonski imunoglobulin, proteinogram, proteomski profil, masni spekter
Work type:Master's thesis (m2)
Typology:2.09 - Master's Thesis
Organization:MF - Faculty of Medicine
Year:2014
Publisher:[K. Jerković]
Number of pages:[VI], 53 str.
PID:20.500.12556/RUL-68758 This link opens in a new window
UDC:543
COBISS.SI-ID:31805657 This link opens in a new window
Publication date in RUL:10.07.2015
Views:1744
Downloads:433
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Secondary language

Language:English
Title:A COMPARISON OF IMMUNOGLOBULIN AND PARAPROTEIN WITH UNUSUAL FEATURE OF PRECIPITATION
Abstract:
When analyzing serum samples in clinical laboratory, we encounter analytical interference. As we mixed serum with sour demineralized water we discovered an interesting phenomenon of precipitation of serum proteins and we soon connected it with monoclonal gammopathy. Appearance of monoclonal immunoglobulin in serum is reflected as a consequence of a group of disease conditions. Because of the unusual features of precipitated proteins and their connection to patology, we named them paraproteins with unusual feature of precipitation. Because proteins do not precipitate in this conditions, we were interested in features and composition of this paraprotein and the reason for its precipitation. Research method In our research we isolated a paraprotein with unusual feature of precipitation and separated it on protein fractions with capillary electrophoresis. With two-dimensional gel electrophoresis we made paraprotein proteomic profile and determined molecular weight of paraprotein’s fragments. With mentioned methods we tried to identify paraprotein and learn as much as possible about its features. Theoretical part of the research is statistical analysis of biomarkers for serum samples with unusual feature of precipitation and samples with monoclonal immunoglobulin without precipitation of paraprotein. Result In our research we found the differences between samples of serum with monoclonal immunoglobulin without precipitation and serums with precipitations of paraprotein. Results of capilaryis electroforesis showed that a larger proportion of paraprotein has a shape of monoclonal immunoglobulin. Immuno complexes on paraprotein proteingrams were not visible. Proteomic profiles of paraprotein are suggesting the presence of heavy chains and light chains of immunoglobulin. Mass spectrum of paraprotein samples showed a mixture of several very large protein molecules, but we couldn't determine molecular weight to larger molecules, due to the limitations of our method.

Keywords:paraprotein, precipitation, monoclonal immunoglobulin, proteinogram, proteomic profile, mass spectrum

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