Nastajanje sekundarne celične stene in lignifikacija traheid kasnega lesa ob kambiju pri navadni jelki (Abies alba) v dormantnem obdobju

Gričar, Jožica (author), Čufar, Katarina (author)

Namen študije je bil proučiti proces diferenciacije terminalnih traheid kasnega lesa ob kambiju v dormantnem obdobju pri 10 navidezno zdravih odraslih jelkah (Abies alba) s pomočjo svetlobne mikroskopije, transmisijske elektronske mikroskopije in UV-mikrospektrofotometrije. Vzorci lesa in kambija so bili odvzeti iz živih dreves v oktobru, novembru in marcu. Kambijeva celična delitvena aktivnost se je pri vseh drevesih zaključila v oktobru, medtem ko je proces diferenciacije v najmlajših traheidah ob kambiju pri drevesih s širšimi branikami še vedno potekal. Sloj S3 sekundarne celične stene ter bradavičasti sloj nista bila oblikovana in notranji deli srednjega sloja S2 sekundarne celične stene še niso bili lignificirani. Štiri tedne kasneje, v novembru, so bile pri vseh drevesih celične stene terminalnih traheid kasnega lesa ob kambiju dokončno oblikovane in lignificirane. Na ultrastrukturni ravni ni bilo opaziti nobenih razlik med novembrskimi ter marčevskimi vzorci. Celična delitvena aktivnost kambija ter diferenciacija sta se pri drevesih s širšimi branikami zaključili kasneje, kot pri drevesih z ožjimi branikami. V vseh proučenih drevesih se je proces diferenciacije v terminalnih traheidah kasnega lesa zaključil pred zimo.

• Language: Slovenian
• Keywords: navadna jelka, traheide kasnega lesa, celična stena, lignifikacija, svetlobna mikroskopija, kalijev permanganat
• Work type: Not categorized
• Typology: 1.16 - Independent Scientific Component Part or a Chapter in a Monograph
• Organization: BF - Biotechnical Faculty
• Publisher: Društvo mladih raziskovalcev Slovenije
• Year: 2004
• Number of pages: Str. 137-143
• PID: 20.500.12556/RUL-68632
• UDC: 630
• COBISS.SI-ID: 1270665
• Publication date in RUL: 10.07.2015

Secondary language

• Language: English
• Title: Secondary Cell Wall Formation and Lignification of Latewood Tracheids near Cambium in Silver Fir (Abies alba) during Dormant Period

Abstract

The aim of this study was to investigate the process of differentiation of terminal latewood tracheids near the cambium in silver firs (Abies alba) during dormant period. Samples of wood and cambium were taken from 10 apparently healthy mature trees in the middle of October, November and March. According to light microscopy, transmission electron microscopy and UV–microspectrophotometry, cambial cell-divisional activity was completed in all investigated trees in October, whereas process of differentiation was still ongoing in the youngest tracheids close to the cambium in trees with broader annual xylem growth rings. Layer S3 of the secondary cell wall and warty layer were not formed and inner parts of S2 layer of secondary cell wall were not yet lignified. Only four weeks later, in November, the cell wall formation and the lignification of terminal latewood tracheids close to the cambium were completed in all trees. No alterations were observed at the ultrastructural level between November and March samples. The cambial cell-divisional activity and also differentiation ended later in trees with broader annual xylem growth rings. However, differentiation of terminal latewood tracheids in all investigated trees was completed before winter.