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Uporaba izbranih svetlobno mikroskopskih tehnik za raziskave lesa in skorje
Prislan, Peter (Author), Merela, Maks (Author), Zupančič, Martin (Author), Krže, Luka (Author), Čufar, Katarina (Author)

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Abstract
Predstavljamo nekaj možnosti uporabe svetlobnega mikroskopa za raziskavo lesa in skorje. Raziskave smo opravili na vzorcih intaktnih tkiv (lesa, kambija, skorje) odvzetih iz odraslih bukev (Fagus sylvatica L.) v različnih obdobjih vegetacijske dobe. Vzorce smo fiksirali in vklopili v parafin ali epoksi smolo po Spurru, pripravili rezine normalnega zrelega lesa in lesa v različnih fazah nastanka, rezine tenzijskega lesa, kambijeve cone ter tkiv skorje. Rezine smo obarvali z različnimi barvili (safranin, astra modro, akridin oranžno in toluidin modro), za primerjavo pa smo pripravili tudi neobarvane rezine. Preparate smo opazovali s svetlobnim mikroskopom in sicer z uporabo različnih tehnik kot so: svetlo polje, polarizacija in UV-fluorescenca. Predstavljamo posnetke različno obarvanih tkiv, opazovanih z različnimi tehnikami ter razpravljamo o prednostih in pomanjkljivostih posameznih tehnik pri uporabah za različne namene.

Language:Slovenian
Keywords:bukev, Fagus sylvatica, les, polarizacija, skorja, svetlo polje, svetlobni mikroskop, UV-fluorescenca
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2009
Publisher:Zveza lesarjev Slovenije: GZS, Združenje lesarstva
Number of pages:str. 222-229
Numbering:Let. 61, št. 5-P
UDC:630*81:176.1 Fagus sylvatica
ISSN on article:0024-1067
COBISS.SI-ID:1735817 Link is opened in a new window
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Downloads:314
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Record is a part of a journal

Title:Les
Shortened title:Les
Publisher:UL, Biotehniška fakulteta, Oddelek za lesarstvo
ISSN:0024-1067
COBISS.SI-ID:13940224 This link opens in a new window

Secondary language

Language:English
Title:Use of selected light microscopy techniques to investigate wood and bark
Abstract:
We present some possibilities to use a light microscope for investigations of wood and bark. We investigated intact tissue samples containing the wood, cambial zone and bark. They were taken from living mature beech (Fagus sylvatica L.) trees at different times of vegetation period. The samples were fixed in FEA (formalin-ethanol-acetic acid) and embedded in paraffin or epoxy resin after Spurr. We made the slices of normal mature wood and of wood in different phases of differentiation, tension wood, cambial zone and bark tissues. The sections were stained with safranin, astra blue, acridine orange and toluidin blue. We also prepared unstained sections for comparison. The sections were observed under the light microscope using bright field and polarization mode, and UV-fluorescence. We present images of sections stained with different stains and observed with different light microscope techniques. We discuss the advantages and disadvantages of all used techniques.


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