Vsebnost celokupnih fenolov v jelovih in smrekovih grčah : diplomsko delo - visokošolski strokovni študij

Sinkar, Boštjan (author), Oven, Primož (mentor), Poljanšek, Ida (comentor), Petrič, Marko (reviewer)

Določevali smo vsebnost ekstraktivov in delež celokupnih fenolov v grčah in okoliškem lesu debla iglavcev. Z napravo po Soxhletu smo najprej ekstrahirali hidrofobne (lipofilne) komponente s cikloheksanom, nato pa še hidrofilne z zmesjo aceton/voda. Delež celokupnih fenolov v grčah in okoliškem lesu iglavcev v acetonskem ekstraktu smo doloŽevali z UV/VIS spektroskopijo. Rezultate smo izrazili v ekvivalentih masne koncentracije monohidrata galne kisline. Delež suhe snovi je bil v grčah 93,33 %, v lesu pa 92,08 %. Lipofilnih in hidrofilnih snovi je bilo v grčah več kot v okoliškem lesu. Povprečni delež celokupnih ekstrahiranih snovi je bil v grčah 14,73 %, v okoliškem lesu pa 2,59 %. Povprečna vsebnost celokupnih fenolov je bila v grčah približno šestkrat večja kot v okoliškem lesu. Fenolne snovi na bazi živih in mrtvih vej (grč) verjetno ščitijo drevesna tkiva pred vdorom patogenih mikroorganizmov po odlomu veje.

• Language: Slovenian
• Keywords: navadna smreka, Picea abies, navadna jelka, Abies alba, grče, Soxhlet, UV-VIS spektroskopija, ekstraktivi, fenoli
• Work type: Undergraduate thesis
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [B. Sinkar]
• Year: 2011
• Number of pages: VIII, 57 f.
• PID: 20.500.12556/RUL-619
• UDC: 630*813.12
• COBISS.SI-ID: 1960841
• Publication date in RUL: 11.07.2014

SINKAR, Boštjan, 2011, Vsebnost celokupnih fenolov v jelovih in smrekovih grčah : diplomsko delo - visokošolski strokovni študij [online]. Bachelor’s thesis. Ljubljana : B. Sinkar. [Accessed 28 May 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=619

Secondary language

• Language: English
• Title: Total phenol content in fir and spruce knot wood : graduation thesis - higher professional studies

Abstract

The main task was to define contents of extractives and portions of entire phenols in wood knots and in its surrounding wood of coniferous trees. We used Soxhlet method device firstly to extract hydrophobic (lipophilic) components with cyclohexane and then to extract hydrophilic components with a mixture of water and acetone. UV/VIS spectroscopy method based on acetone extract was used to define portions of entire phenols in wood knots and in its surrounding wood. The results were presented in equivalents of gallic acid mass concentration. The dry substance share in wood knots was 93.33 %, and in its surrounding wood it was 92.08 %. Lipophilic and hydrophilic substance shares in wood knots was 14.73 %, and in its surrounding wood 2.59 %. The average content of entire phenols was about six times higher in knots than in its surrounding wood. It is assumed that phenol substances protect wood tissue after breaking of branches against irruption of pathogenic microorganisms.

• Keywords: spruce, Picea abies, fir, Abies alba, knots, Soxhlet, UV-VIS spectroscopy, extractives, phenols