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Vsebnost celokupnih fenolov v jelovih in smrekovih grčah : diplomsko delo - visokošolski strokovni študij
Sinkar, Boštjan (Author), Oven, Primož (Mentor) More about this mentor... This link opens in a new window, Poljanšek, Ida (Co-mentor), Petrič, Marko (Reviewer)

URLURL - Presentation file, Visit http://www.digitalna-knjiznica.bf.uni-lj.si/vs_sinkar_bostjan.pdf New window

Abstract
Določevali smo vsebnost ekstraktivov in delež celokupnih fenolov v grčah in okoliškem lesu debla iglavcev. Z napravo po Soxhletu smo najprej ekstrahirali hidrofobne (lipofilne) komponente s cikloheksanom, nato pa še hidrofilne z zmesjo aceton/voda. Delež celokupnih fenolov v grčah in okoliškem lesu iglavcev v acetonskem ekstraktu smo doloŽevali z UV/VIS spektroskopijo. Rezultate smo izrazili v ekvivalentih masne koncentracije monohidrata galne kisline. Delež suhe snovi je bil v grčah 93,33 %, v lesu pa 92,08 %. Lipofilnih in hidrofilnih snovi je bilo v grčah več kot v okoliškem lesu. Povprečni delež celokupnih ekstrahiranih snovi je bil v grčah 14,73 %, v okoliškem lesu pa 2,59 %. Povprečna vsebnost celokupnih fenolov je bila v grčah približno šestkrat večja kot v okoliškem lesu. Fenolne snovi na bazi živih in mrtvih vej (grč) verjetno ščitijo drevesna tkiva pred vdorom patogenih mikroorganizmov po odlomu veje.

Language:Slovenian
Keywords:navadna smreka, Picea abies, navadna jelka, Abies alba, grče, Soxhlet, UV-VIS spektroskopija, ekstraktivi, fenoli
Work type:Undergraduate thesis (m5)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2011
Publisher:[B. Sinkar]
Number of pages:VIII, 57 f.
Place:Ljubljana
UDC:630*813.12
COBISS.SI-ID:1960841 Link is opened in a new window
Views:477
Downloads:157
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Secondary language

Language:English
Abstract:
The main task was to define contents of extractives and portions of entire phenols in wood knots and in its surrounding wood of coniferous trees. We used Soxhlet method device firstly to extract hydrophobic (lipophilic) components with cyclohexane and then to extract hydrophilic components with a mixture of water and acetone. UV/VIS spectroscopy method based on acetone extract was used to define portions of entire phenols in wood knots and in its surrounding wood. The results were presented in equivalents of gallic acid mass concentration. The dry substance share in wood knots was 93.33 %, and in its surrounding wood it was 92.08 %. Lipophilic and hydrophilic substance shares in wood knots was 14.73 %, and in its surrounding wood 2.59 %. The average content of entire phenols was about six times higher in knots than in its surrounding wood. It is assumed that phenol substances protect wood tissue after breaking of branches against irruption of pathogenic microorganisms.

Keywords:spruce, Picea abies, fir, Abies alba, knots, Soxhlet, UV-VIS spectroscopy, extractives, phenols

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