Genetic variation of the mitochondrial D-loop region containing mitochondrial transcription factor (TFAM) binding sites is not associated with marbling in Wagyu X Limousin F2 crosses

Kunej, Tanja (author), Michal, Jennifer J. (author), Dovč, Peter (author), Jiang, Zhihua (author)

Mitochondrial transcription factor A (TFAM) is a nucleus-encoded protein that is essential for initiation of transcription and replication of mitochondrial DNA (mtDNA). It has been shown that TFAM binds the entire length of the mtDNA,but with higher affinitz to sequences around both mitochondrial promoters located in 3' domain of the D-loop region. The objectives of this study were to detect genetic polymorphisms in the sequence flanking TFAM binding sites of the mtDNA D-loop region and investigate their associations with marbling in Wagzu x Limousin F2 crosses. We identified six polymorphis sites in the TFAM binding sites of the D-loop mtDNA (G8A, T106C, A169G, A173G and C190T and one insertion/deletion at position 221 with one or two cytosines) with no significant differences in frequencies of polymorphic sites between extreme high/low marbling pools.

• Language: English
• Keywords: cattle, breeds, Limousin, Wagyu, crosses, molecular genetics, mitochondrial transcription factor, mtDNA, muscles, marbling
• Work type: Not categorized
• Typology: 1.03 - Other scientific articles
• Organization: BF - Biotechnical Faculty
• Publisher: Biotehniška fakulteta
• Year: 2005
• Number of pages: Str. 99-102
• Numbering: Letn. 86, št. 2
• PID: 20.500.12556/RUL-60451
• UDC: 636.2
• ISSN on article: 1581-9175
• COBISS.SI-ID: 1833096
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Shortened title: Acta agric. Slov.
• Publisher: Biotehniška fakulteta
• ISSN: 1581-9175
• COBISS.SI-ID: 213840640

Secondary language

• Language: Slovenian
• Title: Genetska raznolikost mitohondrijske D-loop regije, ki vsebuje vezavno mesto za mitohondrijski transkripcijski faktor A (TFAM), ni povezana z marmoriranostjo pri F2 Wagyu X limousin križancih

Abstract

Mitohondrijski transkripcijski factor A (TFAM) je v jedru kodiran protein, udeležen v iniciaciji transkripcije in replikacije mitohondrijske DNA (mtDNA). Predhodne raziskave so pokazale, da se TFAM veže po vsej dolžini mtDNA, z večjo afiniteto pa na zaporedja v področju mitohondrijskih promotorjev, ki se nahajata na 3' območju D-loop regije. Namen naše študije jebil identificirati genetske polimorfizme v območju vezave TFAM v D-loop regiji mtDNA in raziskati njihovo povezavo z marmoriranostjo misic pri F2 Wagyu x Limousin križancih. V območju vezave TFAM smo v D-loop regiji mtDNA identificirali šest polimorfnih mest (G8A, T106C, A169G, A173G, C190T, insercija/delecija enega ali dveh C na poziciji 221), razporeditev alelov pa se ni statistično značilno razlikovala med skupinama živali z visokimi in nizkimi vrednostmi marmoriranosti.

• Keywords: govedo, pasme, limuzin, wagyu, križanci, molekularna genetika, mitohondrijski transkripcijski faktor, mtDNK mišice, marmoriranost