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Sposobnost biohidrogenacije linolne kisline pri vampni bakteriji Pseudobutyrivibrio xylanivorans Mz5T
ID Čepeljnik, Tadej (Author), ID Devillard, Estelle (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:doc-6UM44NT1 This link opens in a new window

Abstract
Konjugirane linolne kisline (CLA) so skupina pozicijskih in geometrijskih izomer oktadekadienojske kisline (18:2) in imajo vrsto ugodnih učinkov pri ljudeh in živalih. Glavni tvorci CLA so vampne bakterije, predvsem iz rodu Butyrivibrio. Vse več je raziskav, s katerimi se preiskušajo načini za povečanje vsebnosti CLA v prehranskih proizvodih živalskega izvora, kar bi lahko dosegli z uporabo primernih probiotičnih sevov bakterij. Eden izmed njihje tudi Pseudobutyrivibrio xylanivorans Mz5T. Ta sev je sposoben rasti v gojišču, kjer je prisotna linolna kislina (LA, to je prekurzor CLA) vsaj do koncentracije 200 g ml-1. Pri takih pogojih se rast sicer upočasni, a bakterije še vedno ohranijo svojo aktivnost. Po 24 urah gojenja v gojišču z LA proučevani sev izvede biohidrogenacijo LA do trans-vakcenske kisline, ki jo tkivni encim ?9-desaturaza, prisoten v tkivu živali, lahko pretvori nazaj do CLA. Z uporabo seva Mz5T kot probiotika v prehrani živali bi tako lahko povečali kakovost končnih živil živalskega izvora.

Language:Slovenian
Keywords:mikrobiologija, anaerobne bakterije, Pseudobutyrivibrio xylanivorans, konjugirana linolna kislina, biohidrogenacija, vamp
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publisher:Biotehniška fakulteta
Year:2006
Number of pages:Str. 75-81
Numbering:Letn. 88, št. 2
PID:20.500.12556/RUL-59608 This link opens in a new window
UDC:579
ISSN on article:1581-9175
COBISS.SI-ID:1969032 This link opens in a new window
Publication date in RUL:10.07.2015
Views:1935
Downloads:260
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Record is a part of a journal

Title:Acta agriculturae Slovenica
Shortened title:Acta agric. Slov.
Publisher:Biotehniška fakulteta
ISSN:1581-9175
COBISS.SI-ID:213840640 This link opens in a new window

Secondary language

Language:English
Title:Capability of biohydrogenation of linoleic acid in rumen bacterium Pseudobutyrivibrio xylanivorans Mz5T
Abstract:
Conjugated linoleic acids (CLA) are positional and geometrical isomers of octadecadienoic acid (18:2) and have a variety of beneficial effects for the humans and animals. Main producers of CLA are rumen bacteria, mainly from the genus Butyrivibrio. Many researches are directed towards increasing the concentration of CLA in food products of animal origin. This could be achieved also with the application of suitable probiotic strains of bacteria. One of those is also Pseudobutzrivibrio xzlanivorans My5T. This strain is capable to grow in the presence of linoleic acid (LA, ie. the CLA precursor), at least up to concentrations of 200 g LA/ml. Under these conditions, the lag phase is prolonged, and the growth is slowered down, too. Consequently the bacteria retain their activity. After 24 hour incubation in the medium with LA, the studied strain biohydrogenates the LA to trans-vaccenic acid, which can be then transformed back to CLA by tissue ?9-desaturase, which is present in the animal tissue. The strain Mz5T could be used as a probiotic in animal nutrition in order to increase the quality of the food products of animal origin.

Keywords:microbiology, anaerobic bacteria, Pseudobutyrivibrio xylanivorans, conjugated linoleic acid, biohydrogenation, rumen

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