izpis_h1_title_alt

The search for conjugative transposon in rumen bacterium Prevotella bryantii B[down]14
Gorenc, Gregor (Author), Accetto, Tomaž (Author), Avguštin, Gorazd (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:doc-35ZXSUZ3 This link opens in a new window

Abstract
Only few plasmids and bacteriophages have been described to date in ruminal prevotella strains, therefore it appears plausible that the genetic exchange in these organisms must exploit other routes. Large conjugative transposons make possible the gene exchange process in bacteria from the genus Bacteroides, the phylogenetic relatives of ruminal prevotellas. The access to fully or partially finished genome sequences of Bacteroides and Prevotella representatives made possible the search for conserved regions within putative conjugative transposons. Multiple sequence alignment of known and putative conjugative transposon gene sequences of Bacteroides thetaiotaomicron, Prevotella intermedia, Bacteroides fragilis and Tanerella sp. was used to locate partially conserved regions within most preserved conjugative transposition genes, traG, and to construct appropriate degenerated oligonucleotide primers. These were used to amplify genome fragments from ruminal prevotella strains. Sequence analysis of the subcloned PCR products revealed the presence of a hypothetical gene in the genome of Prevotella bryantii B14, similar to the ORF BF2880 from B. fragilis YCH46, which is a part of a large conjugative transposon. Inverse PCRs were designed and performed to confirm the initial findings. A partial map of P. bryantii B14 putative conjugative transposon region was constructed, indicating an intergeneric horizontal gene transfer.

Language:English
Keywords:microbiology, molecular genetics, conjugative transposon, Prevotella bryantii, Bacteroides fragilis
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2009
Publisher:Biotehniška fakulteta
Number of pages:str. 147-152
Numbering:Letn. 94, št. 2
UDC:579
ISSN on article:1581-9175
COBISS.SI-ID:2547592 Link is opened in a new window
Views:595
Downloads:125
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Record is a part of a journal

Title:Acta agriculturae Slovenica
Shortened title:Acta agric. Slov.
Publisher:Biotehniška fakulteta
ISSN:1581-9175
COBISS.SI-ID:213840640 This link opens in a new window

Secondary language

Language:Slovenian
Title:Iskanje konjugativnega transpozona v vampni bakteriji Prevotella bryantii B[down]14
Abstract:
Ker sevi rodu Prevotella iz vampa le izjemoma posedujejo plazmide in je opisanih le nekaj bakteriofagov, je zelo verjetno, da izmenjava genov pri teh organizmih vključuje druge poti. Veliki konjugativni transpozoni omogočajo prenos genov pri rodu Bacteroides, filogenetskih sorodnikih vampnih prevotel. Dostop do delno ali v celoti sekvenciranih genomov predstavnikov Bacteroides in Prevotella je omogočil iskanje ohranjenih regij znotraj domnevnih konjugativnih transpozonov. S poravnavo več sekvenc znanih ali domnevnih genov konjugativnih transpozonov iz vrst Bacteroides thetaiotaomicron, Prevotella intermedia, Bacteroides fragilis in rodu Tanerella smo določili delno ohranjene regije v najbolj ohranjenem genu konjugativne transpozicije, traG, in jih uporabili za izdelavo primernih začetnih oligonukleotidov za pomnoevanje dela gena pri vampnih sevih iz rodu Prevotella. Analiza sekvenc subkloniranih pomnokov je pri P. bryantii B14 razkrila prisotnost hipotetičnega gena, podobnega odprtemu čitalnemu okvirju BF2880 seva B. fragilis YCH46, ki je del velikega konjugativnega transpozona. Z inverzno verižno reakcijo s polimerazo smo potrdili prvotne ugotovitve. Izdelali smo delno mapo regije domnevnega konjugativnega transpozona pri P. bryantii B14, ki nakazuje, da je prišlo do med rodovnega horizontalnega prenosa genov.

Keywords:mikrobiologija, molekularna genetika, konjugativni transpozon, Prevotella brayantii, Bacteroides fragilis

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back