izpis_h1_title_alt

Phototransduction and clock gene expression in the troglobiont beetle Ptomaphagus hirtus of Mammoth cave
Friedrich, Markus (Author), Chen, Rui (Author), Daines, Bryce (Author), Bao, Riyue (Author), Caravas, Jason (Author), Rai, Puneet K. (Author), Zagmajster, Maja (Author), Peck, Stewart B. (Author)

URLURL - Presentation file, Visit http://dx.doi.org/10.1242/jeb.060368 This link opens in a new window

Abstract
Obligatory cave species exhibit dramatic trait modifications such as eye reduction, loss of pigmentation and an increase in touch receptors. As molecular studies of cave adaptation have largely concentrated on vertebrate models, it is not yet possible to probe for genetic universalities underlying cave adaptation. We have therefore begun to study the strongly cave-adapted small carrion beetle Ptomaphagus hirtus. For over 100 years, this flightless signature inhabitant of Mammoth Cave, the worldćs largest known cave system, has been considered blind despite the presence of residual lens structures. Bydeep sequencing of the adult head transcriptome, we discovered the transcripts of all core members of the phototransduction protein machinery. Combined with the absence of transcripts of select structural photoreceptor and eye pigmentation genes, these data suggest a reduced but functional visualsystem in P. hirtus. This conclusion was corroborated by a negative phototactic response of P. hirtus in lightždark choice tests. We further detected the expression of the complete circadian clock gene network in P. hirtus, raising the possibility of a role of light sensation in the regulation of oscillating processes. We speculate that P. hirtus is representative of a large number of animal species with highly reduced but persisting visual capacities in the twilight zone of the subterranean realm. These can now be studied on a broad comparative scale given the efficiency of transcript discovery by next-generation sequencing.

Language:English
Keywords:biospeleology, cave adaptation, circadian clock, phototransduction, evolution
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2011
Number of pages:str. 3532-3541
Numbering:Vol. 214
UDC:591
ISSN on article:0022-0949
DOI:10.1242/jeb.060368 Link is opened in a new window
COBISS.SI-ID:2449743 Link is opened in a new window
Views:422
Downloads:205
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Record is a part of a journal

Title:Journal of Experimental Biology
Shortened title:J. Exp. Biol.
Publisher:Cambridge University Press
ISSN:0022-0949
COBISS.SI-ID:4736266 This link opens in a new window

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back