Introducing mothur : open-source, platform-independent, community-supported software for describing and comparing microbial communities

Schloss, Patrick D. (author), Westcott, Sarah L. (author), Ryabin, Thomas (author), Hall, Justine R. (author), Hartmann, Martin (author), Hollister, Emily B. (author), Lesniewski, Ryan A. (author), Oakley, Brian B. (author), Parks, Donovan H. (author), Robinson, Courtney J. (author), Sahl, Jason W. (author), Stres, Blaž (author), Thallinger, Gerhard G. (author), Horn, David J. van (author), Weber, Caroly F. (author)

Mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur totrim, screen, and align sequences, calculate distances, assign sequences to OTUs, and describe that alfa-and ß-diversity of eight marine samples previously characterized by pyrpsequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 hours using laptop computer.

• Language: English
• Keywords: mikrobiologija, mikrobne združbe, bioinformatika, mothur
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2009
• Number of pages: Str. 7537-7541
• Numbering: Vol. 75, No. 23
• PID: 20.500.12556/RUL-36506
• UDC: 579
• ISSN on article: 0099-2240
• DOI: 10.1128/AEM.0541-09
• COBISS.SI-ID: 2517640
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Applied and environmental microbiology
• Shortened title: Appl. environ. microbiol.
• Publisher: American Society for Microbiology
• ISSN: 0099-2240
• COBISS.SI-ID: 5133319