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Anti-idiotypic antibodies : a new approach in prion research
Colja Venturini, Anja (Author), Bresjanac, Mara (Author), Vranac, Tanja (Author), Koren, Simon (Author), Narat, Mojca (Author), Popović, Mara (Author), Čurin-Šerbec, Vladka (Author)

URLURL - Presentation file, Visit http://www.biomedcentral.com/1471-2172/10/16 This link opens in a new window

Abstract
In certain cases, anti-idiotypic antibodies that recognize an antigen-combining site of an antibody can mimic the structure and/or function of certain nominal antigens. This feature makes them particularly useful if conventional experimental approaches fail to fulfil expectations, especially when the molecule of interest is infectious, toxic or difficult to isolate and purify. We suggest the application of an anti-idiotype concept to the field of prion biology, with the aim of evoking a humoral immune response against the pathological isoform of the prion protein (PrPsc). Different ways to induce anti-idiotypic responses were studied in mice and chickens using various forms of V5B2, a PrPsc-specific monoclonal antibody we have described previously. The preparation of anti-idiotypic monoclonal antibodies was achieved with well-defined strategies of immunization, selection and subsequent characterization. Our results demonstrate that it is possible to induce a strong anti-idiotypic immune response against the V5B2 monoclonal antibody in both xenogeneic and syngeneic experimental systems. From the competition seen between polyclonal and monoclonal anti-iditypic antibodies and the original immunogen, the PI peptide, and even more importantly, the ultimate target antigen, PrPsc, we conclude that selected antibodoes bind to the antigen-combining site of hte V5B2 monoclonal antibody and might even resemble the PrPSc-specific epitope. The involvement of both antigen-combining sites in the interaction between V5B2 and the most promising monoclonal anti-idiotypic antibody was further supported by molecular docking. The results of the present study not only provide an example of the successful production of Ab2 monoclonal antibodies based on a well planned strategy for selection, but should also provide a new experimental approach that is applicable to the field of prion diseases.

Language:English
Keywords:immunologija, anti idiopatska protitelesa, prioni
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2009
Number of pages:on line
Numbering:Vol. 10, article 16
UDC:577.27
ISSN on article:1471-2172
DOI:10.1186/1471-2172-10-16 Link is opened in a new window
COBISS.SI-ID:2440072 Link is opened in a new window
Views:687
Downloads:232
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Record is a part of a journal

Title:BMC immunology
Shortened title:BMC Immunol
Publisher:BioMed Central
ISSN:1471-2172
COBISS.SI-ID:2439188 This link opens in a new window

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