Four selected white-rot fungi (Pleurotus ostreatus, Trametes versicolor, Chondrostereum purpureum, Hypoxylon fragiforme) and one brown-rot fungus (Gloeophyllum trabeum) were analysed for their mycoremediation potential by following the degradation of polychlorinated organic biocides lindane, sodium pentachlorophenol, and PCB-153, respectively. Among selected white-rot fungi, P. ostreatus showed the highest efficiency in degrading lindane and PCB-153, while sodium pentachlorophenol was most efficiently degraded by T. versicolor. White rots C. purpureum and H. fragiforme, and a brown rot G. trabeum were considerably less effective in degrading all the tested biocides. Monitoring of ligninolytic enzymes activities showed the highest expression of laccase, manganese peroxidase, and lignin peroxidase in P. ostreatus and T. versicolor. Temporal expression of ligninolytic enzymes in liquid cultures with addition of lindane, sodium pentachlorophenol, or PCB-153 differed from the negative controls. In comparison to fungal degradation, an application of respective commercially available ligninolytic enzymes laccase, manganese peroxidase, and versatile peroxidase was less efficient in degradation of lindane or chlorinated phenols. Manganese peroxidase and versatile peroxidase were more applicable enzymes in these cases. Inhibition of ABTS oxidation by ligninolytic enzymes in the presence of sodium pentachlorophenol showed that sodium pentachlorophenol may act as a substrate for enzymes manganese peroxidase and versatile peroxidase, and not for laccase.