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Razgradnja kloriranih organskih biocidov z lignolitičnimi glivami : magistrsko delo
Vidic, Iztok (Author), Pohleven, Franc (Mentor) More about this mentor... This link opens in a new window

URLURL - Presentation file, Visit http://www.digitalna-knjiznica.bf.uni-lj.si/md_vidic_iztok.pdf This link opens in a new window

Abstract
Študirali smo razgradnjo kloriranih organskih biocidov lindana, natrijevega pentaklorofenolata in PCB-153 z izbranimi lesnimi glivami, od katerih so bile vrste Pleurotus ostreatus, Trametes versicolor, Chondrostereum purpureum in Hypoxylon fragiforme predstavnice gliv bele trohnobe, vrsta Gloeophyllum trabeum pa sodi v skupino gliv, ki povzročajo rjavo trohnobo. Razgradnja posameznih kloriranih organskih biocidov je bila z glivami bele trohnobe uspešna, pri čemer je največji delež lindana in PCB-153 razgradila gliva P. ostreatus, vrsta T. versicolor pa je uspela popolnoma razgraditi natrijev pentaklorofenolat. Časovno spremljanje aktivnosti posameznih ligninolitičnih encimov lakaze, mangan peroksidaze in lignin peroksidaze je pokazalo, da vrsti P. ostreatus in T. versicolor izrazata veliko večje vrednosti ligninolitičnih encimov, kot ostale testirane vrste gliv. Pri glivah P. ostreatus in T. versicolor, zraslih v prisotnosti posameznih biocidov, je bil vzorec časovnega izražanja ligninolitičnih encimov lakaze, mangan peroksidaze in lignin peroksidaze drugačen, kot pri negativnih kontrolnih skupinah. Proučili smo tudi razgradnjo lindana in kloriranih fenolov s posameznimi komercialnimi ligninolitičnimi encimi (lakazo, mangan peroksidazo in raznoliko peroksidazo), pri čemer sta bili mangan peroksidaza in raznolika peroksidaza v primerjavi z lakazo ustreznejša ligninolitična encima za njihovo razgradnjo. Razgradnja lindana je bila uspešnejša s posameznimi ligninolitičnimi glivami, kot s posameznimi ligninolitičnimi encimi. V prisotnosti natrijevega pentaklorofenolata smo s testi inhibicije oksidacije substrata ABTS s posameznimi komercialnimi ligninolitičnimi encimi ugotovili, da je natrijev pentaklorofenolat potencialen substrat za mangan peroksidazo in za raznoliko peroksidazo, ne pa za lakazo.

Language:Slovenian
Keywords:mikoremediacija, biocidi, poliklorirani bifenili, lindan, natrijev pentaklorofenolat, glive, bela trohnoba, lakaza, mangan peroksidaza, lignin peroksidaza, encimska aktivnost
Work type:Master's thesis (m2)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2008
Publisher:[I. Vidic]
Number of pages:XV, 104 f., [7] f. pril.
Place:Ljubljana
UDC:577.2:582.28(043.2)=163.6
COBISS.SI-ID:1728393 Link is opened in a new window
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Downloads:194
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Secondary language

Language:English
Abstract:
Four selected white-rot fungi (Pleurotus ostreatus, Trametes versicolor, Chondrostereum purpureum, Hypoxylon fragiforme) and one brown-rot fungus (Gloeophyllum trabeum) were analysed for their mycoremediation potential by following the degradation of polychlorinated organic biocides lindane, sodium pentachlorophenol, and PCB-153, respectively. Among selected white-rot fungi, P. ostreatus showed the highest efficiency in degrading lindane and PCB-153, while sodium pentachlorophenol was most efficiently degraded by T. versicolor. White rots C. purpureum and H. fragiforme, and a brown rot G. trabeum were considerably less effective in degrading all the tested biocides. Monitoring of ligninolytic enzymes activities showed the highest expression of laccase, manganese peroxidase, and lignin peroxidase in P. ostreatus and T. versicolor. Temporal expression of ligninolytic enzymes in liquid cultures with addition of lindane, sodium pentachlorophenol, or PCB-153 differed from the negative controls. In comparison to fungal degradation, an application of respective commercially available ligninolytic enzymes laccase, manganese peroxidase, and versatile peroxidase was less efficient in degradation of lindane or chlorinated phenols. Manganese peroxidase and versatile peroxidase were more applicable enzymes in these cases. Inhibition of ABTS oxidation by ligninolytic enzymes in the presence of sodium pentachlorophenol showed that sodium pentachlorophenol may act as a substrate for enzymes manganese peroxidase and versatile peroxidase, and not for laccase.

Keywords:mycoremediation, biocides, polychlorinated biphenyls, lindane, sodium pentachlorphenolate, fungi, white-rot, laccase, manganese peroxidase, lignin peroxidase, enzyme activity

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