The glypican 3-hosted murine Mir717 gene : sequence conservation, seed region polymorphisms and putative targets

Kunej, Tanja (author), Jevšinek Skok, Daša (author), Horvat, Simon (author), Dovč, Peter (author), Jiang, Zhihua (author)

Mir717 (mmu-mir-717) was first reported in mouse and resides in the intron 3 of glypican 3 )Gpc3) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites within the mature seed region in mice. Our findiong represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature RNA seed sibgle nucleotide polymorphism (SNP) identified in the lean mouse strain 129/Sv. Additionally, genes potentialy targeted by Mir717 include 91 genes associated with obesity and realted phenbotypes in mammals. Our analaysis provides a basis for further experiments to casually connect the identified SNP and Mir717 gene itself to obesity regulation. Furthermore, our bioinformatics analysis now enables functional annotation of Mir717 orthologs in other species, thus determining the effect of its target genes on fat-related traits.

• Language: English
• Keywords: molekularna genetika, geni, Mir717
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2010
• Number of pages: Str. 769-772
• Numbering: Vol. 6, no.7
• PID: 20.500.12556/RUL-32833
• UDC: 575
• COBISS.SI-ID: 2780808
• Publication date in RUL: 10.07.2015