The majority of children spend their childhood in children day care centers, which represent the child's physical, biological and social environment. The purpose of this master thesis is to perform integral evaluation of comfort in twenty-five playrooms of eighteen Central-Slovenian children day care centers. Integral evaluation of comfort has been performed in four steps, which included measurements of selected parameters of comfort, as well as surveying of the employees. The comprehensive literature review was carried out in step 1 as we identified the problems and defined criteria for integral evaluation of comfort. In-situ measurements of the selected parameters of thermal and visual comfort, indoor air quality and acoustic comfort were carried out in step 2 (objective evaluation of comfort). In addition, the subjective evaluation of comfort was performed in step 3 on the basis of validated questionnaire. In the context of step 4 – integral evaluation of comfort – we compared the measured and calculated parameters of each comfort type, analyzed the possible relationship between various parameters of comfort in selected playrooms, compared the results of objective and subjective evaluation of comfort and exposed the key conclusions. Based on the implemented steps we formulated integral comfort scale. We suggested possible improvements for achieving comfortable and healthy conditions in children day care centers. We have found out that there are no researches, which would include integral evaluation of thermal, visual, acoustic comfort and indoor air quality of children day care centers. Consequently, we are the first who used integral evaluation of comfort approach in an educational institution. We have discovered that comfort in majority of day care centers is poor, while subjective evaluations of comfort parameters in playrooms were deviating from the objective ones. On the basis of integral comfort scale only two out of twentyfive playrooms were evaluated as integrally comfortable. Both, for new buildings as well as for the renovation of educational institutions, we suggest comprehensive measures, based on the presented integral evaluation of comfort.