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Uporaba DNA označevalcev za preverjanje porekla pri ovcah in določanja očetovstva v čredah z več plemenskimi ovni
ID Volčič, Tomaž (Author), ID Kompan, Drago (Author), ID Dovč, Peter (Author), ID Horvat, Simon (Author)

URLURL - Presentation file, Visit http://aas.bf.uni-lj.si/zootehnika/94-2009/PDF/94-2009-2-121-131.pdf This link opens in a new window

Abstract
Rodovniški podatki kontroliranih tropov so bistvenega pomena za rejce ovc in izvajanje selekcijskih programov. V tej študiji smo preverili, če je mono z osmimi molekularno genetskimi označevalci ter uporabo računalniškega programa ATLAS preveriti skladnost podatkov v več-generacijskih rodovnikih jezersko-solčavske in oplemenjene jezersko-solčavske pasme. Ugotovili smo 90,9% točnost beleženja rodovniških podatkov, medtem ko smo neskladja ugotovili pri štirih družinah od 44-ih. V dveh primerih je bil potomcu nepravilno pripisan oče ali mati, pri ostalih dveh primerih pa sta bila jagnjetu napačno pripisana oba starša. V drugem delu raziskave smo poskušali z istim nizom mikrosatelitnih označevalcev določiti očetovstva štirim jagnjetom istrske pramenke, kjer v času paritev v trop pripuščajo dvajset ovnov in tako jagnje nima poznanega očeta. Trem jagnjetom smo lahko nedvoumno določili očeta z izločitvijo ostalih devetnajstih. V enem primeru smo ugotovili, da niz osmih označevalcev ni dovolj informativen za določitev očeta potomcu, zato smo analizo razširili in z vključitvijo dodatnih štirih mikrosatelitnih označevalcev uspešno določili očetovstvo tudi v tem primeru. Z izbranim nizom molekularnih označevalcev in obdelave podatkov je mono učinkovito preverjati obstoječe rodovniške podatke in napovedati očetovstvo jagnjetom v tropih, kjer pripuščajo večje število ovnov. Rezultati take analize so lahko v pomoč rejcem in selekcijskim službam za izboljšanje točnosti rodovniških podatkov in učinkovitejše načrtovanje reje in selekcije pri ovcah.

Language:Slovenian
Keywords:ovce, poreklo, očetovstvo, molekularna genetika, genetski označevalci
Work type:Not categorized (r6)
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2009
Number of pages:Str. 121-131
Numbering:Letn. 94, št. 2
PID:20.500.12556/RUL-321 This link opens in a new window
UDC:636.3:575
ISSN on article:1581-9175
COBISS.SI-ID:2545800 This link opens in a new window
Publication date in RUL:11.07.2014
Views:2007
Downloads:288
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Record is a part of a journal

Title:Acta agriculturae Slovenica
Shortened title:Acta agric. Slov.
Publisher:Biotehniška fakulteta
ISSN:1581-9175
COBISS.SI-ID:213840640 This link opens in a new window

Secondary language

Language:English
Abstract:
Pedigree data from recorded flocks are of importance for the proper flock management as well as for the selection programmes and accurate performance prediction. In this study we examined if the use of eight molecular markers and computer analysis package ATLAS can be applied to verify known pedigrees of Jezersko-Solčava and Improved Jezersko-Solčava sheep breed from Slovenia. 90.9 % of pedigree data were in concordance with molecular genetic tests whereas in four pedigrees discordant parentage tests were obtained. In two cases, a different father or mother was assigned, whereas in the other two pedigrees both parents were discordant with molecular test results. In the second part of this study we aimed to determine the paternity for four lambs of Istrian Pramenka breed, in which a random mating scheme with 20 rams was used and hence the lambs had no father assigned. Using the same set of eight microsatellite markers, we were able to unequivocally determine paternity for 3 out of 4 lambs. In one case the analysis was not informative enough but with inclusion of 4 more microsatellite markers its sire could be determined. With the chosen set of microsatellite markers and data analysis programme ATLAS it is therefore possible to efficiently perform pedigree data validation as well as paternity prediction for lambs from flocks, where a large number of rams are used in a random mating system. Applying such molecular tests could help sheep breeders in flock management and improve efficiency of selection programmes.

Keywords:sheep, parentage, paternity, molecular genetics, genetics markers

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