Določitev galne kisline, pirokatehola, floroglucinola, resorcinola in katehinas tankoplastno kromatografijo

Poljanšek, Ida (author), Vek, Viljem (author), Oven, Primož (author)

Preučevali smo primernost šestih različnih zmesi topil - razvijalcev za ločitev enostavnih fenolnih spojin, kot so galna kislina, pirokatehol, floroglucionol, resorcinol, in sorodno spojino katehin s pomočjo tankoplastne kromatografije. Kot najbolj primerna sistema topil za ločitev komponent na stacionarni fazi silikagela na aluminijasti foliji sta se izkazala kloroform -etilacetat - ocetna kislina (prostorninsko razmerje; 50 : 50 : 1) in toluen -acetonitril - mravljična kislina (prostorninsko razmerje; 70 : 30 : 1). Ločitev organskih spojin je bila učinkovitejša v sistemih topil, ki so imeli večji delež organskih topil. Spojine smo vizualizirali s pomočjo dveh reagentov, in sicer z železovim(III) kloridom in z vanilin-žveplovim reagentom.

• Language: Slovenian
• Keywords: les, estraktivi, fenolne spojine, katehin, TLC
• Work type: Scientific work
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2011
• Number of pages: Str. 3-9
• Numbering: Št. 95
• PID: 20.500.12556/RUL-28750
• UDC: 630*84(045)=163.6
• ISSN on article: 0351-3114
• COBISS.SI-ID: 3256486
• Publication date in RUL: 11.07.2014

Record is a part of a journal

• Title: Zbornik gozdarstva in lesarstva. forest and wood science & technology
• Shortened title: Zb. gozd. lesar.
• Publisher: Gozdarski inštitut Slovenije, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
• ISSN: 0351-3114
• COBISS.SI-ID: 6206978

Secondary language

• Language: English

Abstract

The suitability of six solvent systems for the thin-layer chromatographic separation of simple phenolic compounds such as gallic acid, pyrocatechol phloroglucinol, resorcinol and related compound catechin were investigated. The most appropriate developing solvent systems for the separation of these organic compounds were chloroform - ethyl acetate - acetic acid (50 : 50 : 1) and toluene -acetonitrile - formic acid (70 : 30 : 1). The best separation of the five organic compounds was achieved using solvents with higher organic content. Detection was carried out using ferric chloride reagent and vanillin-sulphuric reagent.