Egerolizini kot označevalci zgodnje apoptoze in kot teranostiki v raziskavah raka sečnega mehurja : raziskovalni podatki, obravnavani v doktorskem delu

Žeželj, Luka (author)

Doktorsko delo preučuje specifične interakcije egerolizinskih proteinov z različnimi lipidnimi domenami ter preverja njihov teranostični potencial. V okviru raziskave so nastali in se zbirali štirje glavni tipi podatkov. Numerični podatki zajemajo eksperimentalne meritve vezave, citotoksičnosti in sproščanja kalceina. Slikovni podatki vključujejo rezultate mikroskopije in grafike. Tekstovni podatki obsegajo zapiske poteka poskusov in rezultate analiz v besedilnih datotekah. Referenčni podatki so pridobljeni iz javnih zbirk kot so PDB, UniProt in PubChem ter vsebujejo podatke o strukturah proteinov in lipidov ter sekvencah v formatih PDB in FASTA. Novi eksperimentalni podatki so bili pridobljeni z nadzorovanimi laboratorijskimi poskusi in specializirano opremo. Uporabljena je bila površinska plazmonska resonanca za pridobivanje podatkov o kinetiki in specifičnosti vezave proteinov. Izvedeni so bili testi na in vitro modelih, in sicer testi selektivne citotoksičnosti za oceno permeabilizacije in viabilnosti celic. Za vizualizacijo vezave na membrane je bila uporabljena fluorescenčna mikroskopija, za visokoločljivostno opazovanje lipidnih veziklov pa transmisijska elektronska mikroskopija. Dodatno so bile izvedene fizikalno-kemijske analize, natančneje CD-spektropolarimetrija za analizo sekundarne strukture proteinov in meritve fluorescenčne anizotropije za preučevanje lastnosti lipidnih membran. Javno dostopni podatki o lipidih in proteinih so pri tem služili kot referenčna osnova, s katero so se neposredno primerjali na novo pridobljeni eksperimentalni rezultati.

• Language: Slovenian
• Keywords: apoptoza, biooznačevalci, egerolizini, kardiolipin, lipidne domene, sfingomielin, teranostika, urotelijski rak
• Work type: Research data
• Typology: 2.20 - Research data
• Geographic coverage: Oddelek za biologijo, Biotehniške fakulteta, Univerza v Ljubljani, Slovenija; Inštitut za biologijo celice, Medicinska fakulteta, Univerza v Ljubljani, Slovenija / Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia; Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Slovenia
• Temporal coverage: Od/From 2022-10-01 do/to 2026-07-30
• Organization: BF - Biotechnical Faculty; MF - Faculty of Medicine
• Year: 2026
• PID: 20.500.12556/RUL-189680
• Data col. methods: Experiment: Laboratory; Synthesis; Measurements and tests
• Publication date in RUL: 12.10.2026

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: English
• Title: Aegerolysins as markers of early apoptosis and theranostics in bladder cancer research : research data underlying the doctoral dissertation

Abstract

The doctoral thesis investigates specific interactions of aegerolysin proteins with various lipid domains and evaluates their theranostic potential. Within the scope of the research, four main types of data were generated and collected. Numerical data include experimental measurements of binding, cytotoxicity, and calcein release. Image data comprise microscopy results and graphics. Textual data encompass notes on experimental procedures and analysis results in text files. Reference data were obtained from public databases such as PDB, UniProt, and PubChem, and include information on protein and lipid structures as well as sequences in PDB and FASTA formats. New experimental data were acquired through controlled laboratory experiments using specialized equipment. Surface plasmon resonance was employed to obtain data on the kinetics and specificity of protein binding. In vitro tests were performed, specifically selective cytotoxicity assays to evaluate cell permeabilization and viability. Fluorescence microscopy was utilized to visualize binding to membranes, while transmission electron microscopy was used for high-resolution observation of lipid vesicles. In addition, physicochemical analyses were conducted, namely CD spectropolarimetry to analyze the secondary structure of proteins and fluorescence anisotropy measurements to study the properties of lipid membranes. Publicly available data on lipids and proteins served as a reference baseline against which the newly obtained experimental results were directly compared.

• Keywords: apoptosis, biomarkers, aegerolysins, cardiolipin, lipid domains, sphingomyelin, theranostics, urothelial cancer