The aim of this thesis was to test the performance of Raman spectroscopy on various organic samples. Raman spectroscopy enables the precise identification of individual organic molecules and, consequently, also the differentiation of microorganisms. In the first part of my diploma, the basic theory of Raman spectroscopy is presented, and its physical principle of operation is explained. The following section describes the methodology uses, including the course of the experimental work, the measurement equipment used, and the processing of the measurements. In the final part, the measured Raman spectra of different samples are compared with reference or data available from the literature, on the basis of which conclusions were drawn about the usefulness of this approach for the identification of organic molecules and microorganisms.
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