This master’s thesis presents the fabrication and analysis of a digital microfluidic system based on electrowetting on dielectric. A droplet is enclosed between two parallel plates, and its movement across an electrode array is controlled by an algorithm. The theoretical part covers the fundamentals of wetting, materials and fabrication techniques, device structure, and selected applications. The experimental part describes electrode fabrication by laser photolithography and the deposition of dielectric and hydrophobic layers by spin coating. The mounting and adjustment of the top plate, which was also evaluated independently, are presented together with the experimental setup and control method. The operation of the closed system was confirmed by performing different droplet manipulation operations. Tests with various droplet volumes reached a velocity of 100 mm/s at 175 V, while the minimum voltage required for movement was 140 V. With suitable operating parameters, repeated droplet merging and splitting were achieved. Droplet dynamics during these operations were observed, and the associated phenomena are discussed. The work provides a basis for further development of closed digital microfluidic systems and more complex configurations.
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