Vpliv načina zamreževanja na koncentracijo kalcijevih ionov v alginatnem hidrogelu

Jesenko, Neja (author), Kopač, Tilen (mentor)

V diplomskem delu so bili pripravljeni alginatni hidrogeli s štirimi različnimi načini ionskega zamreženja: zunanjim zamreženjem s kapljanjem in pršenjem, notranjim zamreženjem s spremembo pH ob prisotnosti slabo topne kalcijeve soli ter predzamreženjem pri 3D biotisku. Koncentracija kalcijevih ionov v pripravljenih hidrogelih je bila določena z metodo optične emisijske spektrometrije z induktivno sklopljeno plazmo (ICP-OES). Rezultati so pokazali, da način zamreženja pomembno vpliva na koncentracijo kalcijevih ionov v hidrogelih. Iz izračunanih afinitetnih parametrov je bilo ugotovljeno, da se z naraščanjem začetne koncentracije kalcijevih ionov relativni delež vgrajenih kalcijevih ionov zmanjšuje, pri čemer so bile najvišje vrednosti afinitetnega parametra dosežene pri hidrogelih, pripravljenih z metodo 3D biotiskanja z ionskim predzamreženjem. Dobljeni rezultati prispevajo k boljšemu razumevanju vpliva načina zamreženja na lastnosti alginatnih hidrogelov.

• Language: Slovenian
• Keywords: hidrogel, ionsko zamreženje, alginat, afiniteta
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2026
• PID: 20.500.12556/RUL-186514
• COBISS.SI-ID: 291380227
• Publication date in RUL: 02.09.2026

Secondary language

• Language: English
• Title: Effect of crosslinking method on calcium ion concentration in alginate hydrogel

Abstract

Alginate hydrogels were prepared using four different ionic crosslinking methods: external crosslinking by dripping and spraying, internal crosslinking induced by a change in pH in the presence of a sparingly soluble calcium salt, and pre-crosslinking during 3D bioprinting. The concentration of calcium ions in the prepared hydrogels was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed that the crosslinking method significantly affects the concentration of calcium ions in the hydrogels. Based on the calculated affinity parameters, it was found that the relative fraction of incorporated calcium ions decreases with increasing initial calcium ion concentration, with the highest affinity parameter values obtained for hydrogels prepared by 3D bioprinting with ionic pre-crosslinking. The obtained results contribute to a better understanding of the influence of the crosslinking method on the properties of alginate hydrogels.

• Keywords: hydrogel, ionic crosslinking, alginate, affinity