Določanje števila veznih mest bakteriofaga T4 s kromatografskim nosilcem

Kante, Ariana (author), Podgornik, Aleš (mentor)

Za diplomsko nalogo sem proučevala vezavo bakteriofaga T4 na QA polyHIPE kromatografski nosilec. Namen naloge je bil določiti število veznih mest bakteriofaga T4 na nosilcu, pri čemer sem kot modelni protein uporabila protein BSA. Eksperimentalni del naloge je bil izveden na HPLC sistemu s pomočjo ionsko-izmenjevalne kromatografije z gradientno elucijo. Rezultate sem analizirala po Yamamoto modelu. Rezultati so pokazali večje število veznih mest pri fagu T4 kot pri proteinu, kar je pričakovano, saj ima fag zaradi večje in kompleksnejše površine več potencialnih mest za vezavo na nosilec.

• Language: Slovenian
• Keywords: protein BSA, bakteriofag T4, ionsko izmenjevalna kromatografija, Yamamoto model
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2026
• PID: 20.500.12556/RUL-185775
• COBISS.SI-ID: 289866243
• Publication date in RUL: 20.08.2026

Secondary language

• Language: English
• Title: Determination of number of binding sites with chromatographic support for bacteriophage T4

Abstract

For my thesis, I studied the binding of bacteriophage T4 to a QA polyHIPE chromatography support. The aim of the of the study was to determine the number of binding sites of phage T4 on the support, using BSA as a model protein. The experimental part was performed on a HPLC system using gradient elution IEX. The results were analysed with Yamamoto model. The results showed a higher number of binding sites for phage T4 than for the protein, which was expected, as the phage has a larger and more complex surface with more potential binding sites on the support.

• Keywords: protein BSA, bacteriophage T4, ion-exchange chromatography, Yamamoto model