Histopathological analysis of the inhibitory effect of ibuprofen on amyloidogenesis in a mouse model

Moghaddam, Atieh Seyedian (author), Azizzadeh, Mohammad (author), Movasseghi, Ahmad Reza (author)

Amyloidosis represents a group of diseases, all characterized by the deposition of protein fibrils with a β-sheet structure. To evaluate the effects of ibuprofen on the inhibition of amyloid deposition, we used an animal model of experimental amyloid A amyloidosis. BALB/c mice (n = 30) were divided into two groups (n = 15 mice/group). Both the treatment and control groups received casein solution for 3 weeks to induce experimental amyloidosis, and ibuprofen was added to the drinking water of the treatment group simultaneously. After 3 weeks, all mice were euthanized. Tissue samples of various organs were collected, fixed, and processed for routine histopathological examination. This revealed amyloid deposition as an amorphous, homogenous, and eosinophilic substance in the control group’s renal glomeruli, hepatic parenchyma, and splenic vascular walls. In the treatment group, only mild amyloid deposition was observed in renal glomeruli and occasionally in the vascular wall of other tissues. The amount of amyloid deposition in the liver and kidneys in the treatment group was significantly lower than in the control group. Our results suggest that ibuprofen may prevent amyloid formation and accumulation in mice, especially in the kidneys and liver.

• Language: English
• Keywords: amyloid A, amyloidosis, casein, ibuprofen, mouse model
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2025
• Year: 2025
• Number of pages: Str. 269-276
• Numbering: Vol. 62, iss. 4
• PID: 20.500.12556/RUL-185531
• UDC: 616.091.8:616:615.2: 599.323.451
• ISSN on article: 2385-8761
• DOI: 10.26873/SVR-1901-2024
• COBISS.SI-ID: 285591555
• Publication date in RUL: 07.08.2026

Record is a part of a journal

• Title: Slovenian veterinary research
• Publisher: Veterinarska fakulteta, Veterinarska fakulteta, Založba Univerze v Ljubljani
• ISSN: 2385-8761
• COBISS.SI-ID: 275836160

Licences

• License: CC BY-NC-SA 4.0, Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-sa/4.0/
• Description: A Creative Commons license that bans commercial use and requires the user to release any modified works under this license.

Secondary language

• Language: Slovenian
• Title: Histopatološka analiza zaviralnega učinka ibuprofena na amiloidogenezo na mišjem modelu

Abstract

Amiloidoza predstavlja skupino bolezni, za katere je značilno odlaganje beljakovinskih fibril s strukturo β-ploskve. Za oceno učinkov ibuprofena na zaviranje odlaganja amiloida smo uporabili živalski model eksperimentalne amiloidoze amiloida A. Miši BALB/c (n = 30) smo razdelili v dve skupini (n = 15 miši/skupina). Tako zdravljena kot kontrolna skupina sta tri tedne prejemali raztopino kazeina za indukcijo eksperimentalne amiloidoze, hkrati pa je bil pitni vodi zdravljene skupine dodan ibuprofen. Po treh tednih so vse miši evtanazirali. Vzorci tkiva različnih organov so bili zbrani, fiksirani in obdelani za rutinsko histopatološko preiskavo. Ta je pokazala odlaganje amiloida kot amorfne, homogene in eozinofilne snovi v ledvičnih glomerulih, jetrnem parenhimu in žilnih stenah vranice kontrolne skupine. V skupini, ki je bila zdravljena, je bilo v ledvičnih glomerulih in občasno v žilni steni drugih tkiv opaženo le blago nalaganje amiloida. Količina odloženega amiloida v jetrih in ledvicah v tej skupini je bila bistveno manjša kot v kontrolni skupini. Naši rezultati kažejo, da lahko ibuprofen prepreči nastajanje in kopičenje amiloida pri miših, zlasti v ledvicah in jetrih.

• Keywords: amiloid A, amiloidoza, kazein, ibuprofen, mišji model