Tea tree (Melaleuca alternifolia) and geranium (Pelargonium graveolens) essential oils as new anesthetics in rainbow trout (Oncorhynchus mykiss)

Didinen, Hakan (author)

In the present study was investigated anesthetic and histopathological effects of tea tree (Melaleuca alternifolia) and geranium (Pelargonium graveolens) essential oils in rainbow trout. These essential oils were applied at 20, 50, 70, 100, 150, 200, 300, 400, 500 and 600 mg L-1 concentrations to determine the anesthetic effect. As a result of this study, major components were found as beta-citronellol (27.52%) for geranium oil and 4-terpineol (40.77%) for tea tree oil. The lowest effective doses of geranium and tea tree oils were 400 mg L-1 and 300 mg L-1 for deep anesthesia of fish, respectively. At these doses, anesthesia induction and recovery times were 129.00 and 247.00 s for geranium essential oil, 118.67 and 65s for tea tree essential oil, respectively. No histopathological findings in liver, kidney and gill tissues were observed in anesthetized fish for each essential oil. In conclusion, geranium and tea tree oils can use as safe and effective anesthetics for rainbow trout.

• Language: English
• Keywords: Oncorhynchus mykiss, anesthesia, histopathology, tea tree, geranium
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2025
• Year: 2025
• Number of pages: Str. 223-231
• Numbering: Vol. 62, iss. 3
• PID: 20.500.12556/RUL-185486
• UDC: 665.52:612.887:639.21
• ISSN on article: 2385-8761
• DOI: 10.26873/SVR-1809-2023
• COBISS.SI-ID: 286362627
• Publication date in RUL: 06.08.2026

Record is a part of a journal

• Title: Slovenian veterinary research
• Publisher: Veterinarska fakulteta, Veterinarska fakulteta, Založba Univerze v Ljubljani
• ISSN: 2385-8761
• COBISS.SI-ID: 275836160

Licences

• License: CC BY-SA 4.0, Creative Commons Attribution-ShareAlike 4.0 International
• Link: http://creativecommons.org/licenses/by-sa/4.0/
• Description: This Creative Commons license is very similar to the regular Attribution license, but requires the release of all derivative works under this same license.

Secondary language

• Language: Slovenian
• Title: Imunohistokemične in histomorfometrične spremembe tankega črevesa, povezane z dodatkom natrijevega butirata pri piščancih

Abstract

Študija je preučevala anestetične in histopatološke učinke eteričnih olj čajevca (Melaleuca alternifolia) in pelargonije (Pelargonium graveolens) na šarenko. Ti eterični olji sta bili uporabljeni v koncentracijah 20, 50, 70, 100, 150, 200, 300, 400, 500 in 600 mg L-1 za določitev anestetičnega učinka. Na podlagi te študije so bile ugotovljene naslednje glavne sestavine: beta-citronelol (27,52 %) za olje geranije in 4-terpineol (40,77 %) za olje čajevca. Najnižji učinkoviti odmerki olja geranije in čajevca so bili 400 mg L-1 oziroma 300 mg L-1 za globoko anestezijo rib. Pri teh odmerkih sta bila čas indukcije anestezije in čas prebujanja iz anestezije 129,00 in 247,00 s za eterično olje geranije ter 118,67 in 65 s za eterično olje čajevca. Pri anesteziranih ribah pri nobenem eteričnem olju niso opazili histopatoloških sprememb v tkivih jeter, ledvic in škrg. Iz tega sledi, da se olji geranije in čajevca lahko uporabljata kot varna in učinkovita anestetika za šarenko.

• Keywords: Oncorhynchus mykiss, anestezija, histopatologija, čajevec, pelargonija