In this master’s thesis study, we aimed to determine how late winter pruning (LWP) and Double Maturation Raisonné (DMR) affect grapevine phenology and grape quality. The experiment was conducted in 2024 on the 'Merlot' variety, in which we included four treatments: late winter pruning on April 22 (LWP-1), late winter pruning on May 1 (LWP-2), Double Maturation Raisonné (DMR), and a control (C). During the growing season, we monitored vine phenology according to the BBCH scale for each treatment, and at harvest, the total number of clusters per vine was recorded. Berries were sampled twice during ripening; for each sample, the weight of 100 berries was measured, the CIRG-index was calculated, and the content of total soluble solids, individual sugars, total and individual acids, pH, as well as total and individual phenolic compounds in berries and seeds were measured. The results showed that LWP delayed vine phenology by an average of 7–10 days, reduced the number of clusters (LWP-2 had approximately four fewer clusters per vine), and decreased the weight of 100 berries (only at the first sampling, by 20–25 g). Grapes from LWP-1 vines had a significantly higher total soluble solids content (21.7±0.18 °Brix) compared to LWP-2 (20.9±0.18 °Brix). The total acidity in grapes from LWP-1 (7.32±0.19 g/L) and LWP-2 (7.33±0.19 g/L) was significantly higher than in the control (5.49 ± 0.19 g/L), but only at the first sampling. Similarly, grapes from LWP-1 and LWP-2 had lower pH values than the control at the first sampling, while at the second sampling, LWP-2 grapes exhibited an even higher pH than the control. The content of total phenols in seeds was significantly higher only in LWP-2, whereas phenolic compounds in berry skins did not differ significantly between treatments. DMR affected only lower weight of 100 berries (by 20–25 g) and higher total soluble solids by 2 to 3 °Brix. LWP is recommended as a practice for delaying phenological development and preserving freshness in wine, while DMR is recommended for increasing total soluble solids concentration and maintaining total acidity.
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