Identification of potential biomarkers for colorectal cancer in a mouse model using proteomics approaches : research data underlying the doctoral dissertation

Sinožić, Tea (author)

Early inflammation-driven tumorigenesis causes significant remodeling of the colonic extracellular matrix, making its proteomic composition an underexplored source of biomarker candidates. In this study, we characterized the proteomes of healthy and cancerous decellularized mouse colons and compared them to native tissues, confirming the decellularization enhances extracellular matrix protein detection. Colitis-associated colorectal cancer was modeled using the azoxymethane and dextran sulfate sodium (AOM/DSS) mouse model. Colon tissues were decellularized and analyzed using mass spectrometry. Proteomic profiling of decellularized tissues identified 26 proteins upregulated in the AOM/DSS group. Among these, three proteins were not previously proposed as stool biomarkers. Two candidates, neutrophilic granule protein and interferon-induced transmembrane protein 3, were confirmed as upregulated in mice stool samples using ELISA. Their performance characteristics were evaluated against clinically used stool biomarkers, haemoglobin and faecal calprotectin, showing complementary results in multi-protein panels for discriminating between early tumorigenic stages and healthy controls, and in discerning inflammation-only changes. These findings demonstrate that decellularized colon proteomics are highly valuable for identifying previously unrecognized stool-detectable proteins that show potential as non-invasive early colorectal cancer biomarkers.

• Language: English
• Keywords: extracellular matrix, decellularization, proteomics, stool biomarker, colorectal cancer, AOM/DSS, mouse model
• Typology: 2.20 - Complete scientific database of research data
• Time coverage: 2021-2026
• Organization: MF - Faculty of Medicine
• Year: 2026
• PID: 20.500.12556/RUL-184286
• Data col. methods: Experiment; Experiment: Laboratory; Measurements and tests: Physical

Note

The 2,078 listed files are organized into the following folders / Navedenih 2.078 datotek je organiziranih v naslednje mape: - AOMDSS experiments, - Decellularization, - ELISA experiments, - SDS PAGE gels for MS, - Western blot experiments.

• Publication date in RUL: 14.07.2026

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Title: Odkrivanje možnih biooznačevalcev raka debelega črevesa in danke pri mišjem modelu s proteomskimi pristopi : raziskovalni podatki, obravnavani v doktorskem delu

Abstract

Zgodnja tumorigeneza, ki jo povzroča vnetje, povzroča znatno preoblikovanje zunajceličnega matriksa debelega črevesa, zaradi česar njegova proteomska sestava predstavlja primeren vir za odkrivanje možnih bioloških označevalcev. V tej študiji smo okarakterizirali proteome zdravih in rakavih decelulariziranih mišjih debelih čreves ter jih primerjali z nativnimi tkivi, s čimer smo potrdili, da decelularizacija izboljša zaznavanje proteinov zunajceličnega matriksa. S kolitisom povezan rak debelega črevesa in danke smo povzročili na mišjem modelu z uporabo azoksimetana in natrijevega dekstran sulfata (AOM/DSS). Tkiva debelega črevesa smo decelularizirali in analizirali z masno spektrometrijo. S proteomskim profiliranjem decelulariziranih tkiv smo identificirali 26 beljakovin, ki so bili povišano izraženi v skupini AOM/DSS. Med temi, trije proteini predhodno niso bili predlagani kot biooznačevalci v blatu. Za dva kandidata, nevtrofilni granularni protein in z interferonom induciran transmembranski protein 3, je bilo s testom ELISA potrjeno, da sta v vzorcih blata miši povečano izražena. Njune zmogljivostne značilnosti smo ovrednotili v primerjavi s klinično uporabljenimi biooznačevalci v blatu, hemoglobinom in fekalnim kalprotektinom, pri čemer so večproteinski paneli pokazali komplementarne rezultate pri razlikovanju med zgodnjimi tumorigenimi stadiji in zdravimi kontrolami ter pri zaznavanju sprememb, ki so posledica zgolj vnetja. Te ugotovitve dokazujejo, da je proteomska analiza decelulariziranega debelega črevesa zelo dragocena za prepoznavanje predhodno neznanih proteinov, zaznavnih v blatu, ki kažejo potencial kot neinvazivni zgodnji biooznačevalci raka debelega črevesa in danke.

• Keywords: zunajcelični matriks, decelularizacija, proteomika, biooznačevalec v blatu, rak debelega črevesa in danke, AOM/DSS, mišji model

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Funding programme: Young Researchers