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Kvalitativna analiza potencialnih biološko aktivnih spojin v prehranskih dopolnilih z maco (Lepidium meyenii) s tekočinsko kromatografijo, sklopljeno z masno spektrometrijo
ID Baković, Ilma (Author), ID Tavčar, Eva (Mentor) More about this mentor... This link opens in a new window, ID Ravnikar, Matjaž (Comentor)

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Abstract
Uravnotežena prehrana je ključna za zdravje, prehranska dopolnila (PD) pa so namenjena dopolnjevanju in izpolnjevanju osnovnih prehranskih potreb. PD vsebujejo različne prehranske sestavine, med katerimi so pogosti rastlinski izvlečki (1). Med njimi se pogosto znajdejo tudi taki, ki vsebujejo spojine z jasnim, klinično dokazanim vplivom na zdravje ali pa še nedokazanim potencialom za to. Naš namen je bil kvalitativno opredeliti prisotnost potencialno biološko aktivnih spojin v prehranskih dopolnilih z mako, dostopnih na slovenskem trgu, ter oceniti skladnost med deklariranimi trditvami in dejansko kemijsko sestavo analiziranih izdelkov. Maka (Lepidium meyenii) je tradicionalna perujska rastlina, ki jo danes največ pridelujejo na Kitajskem (3). Pregledali smo slovenski trg in nabavili 10 PD, katerih glavna rastlina je maka. Na podlagi pregleda znanstvene literature smo pripravili nabor spojin, ki naj bi bile odgovorne za posamezne učinke make, in z uporabo UHPLC-HRMS analizirali vzorce. Podatke smo obdelali s programom Xcalibur FreeStyle. Analiza je pokazala, da se deklarirane trditve o potencialnem delovanju izdelkov večinoma ujemajo z dejansko prisotnostjo spojin. Čeprav je bila v našo analizo zajeta 101 potencialna spojina, smo v vzorcih zaznali približno 20 aktivnih komponent. Zaznane spojine so skladne z učinki, ki se za mako navajajo v literaturi in pri oglaševanju izdelkov, kot so zmanjšanje utrujenosti, izboljšanje razpoloženja in vpliv na plodnost. Rezultati kažejo, da PD z mako vsebujejo spojine, ki lahko prispevajo k navedenim učinkom, vendar njihova prisotnost še ne dokazuje klinične učinkovitosti, temveč le biološko osnovo za oglaševane trditve. Naša raziskava predstavlja osnovo za nadaljnje kvantitativne raziskave, v katerih bi z uporabo standardnih referenčnih spojin vsebnost analizirali še kvantitativno in tako ugotovili, ali je količina priporočenih dnevnih odmerkov obravnavanih PD varna in dovolj velika za dosego klinične učinkovitosti. Na osnovi rezultatov te magistrske naloge lahko zaključimo, da se kemijska sestava prehranskih dopolnil z mako med izdelki razlikuje, zaznane spojine pa le delno podpirajo deklarirane trditve o njihovem delovanju.

Language:Slovenian
Keywords:Lepidium meyenii, UHPLC-MS, kvalitativna analiza
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FFA - Faculty of Pharmacy
Publisher:[I. Baković]
Year:2026
PID:20.500.12556/RUL-183526 This link opens in a new window
UDC:543.061(043.2)
COBISS.SI-ID:281597187 This link opens in a new window
Publication date in RUL:13.06.2026
Views:49
Downloads:33
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Secondary language

Language:English
Title:Qualitative analysis of potential bioactive compounds in food supplements containing maca (Lepidium meyenii) using liquid chromatography coupled with mass spectrometry
Abstract:
A balanced diet is essential for maintaining health, while food supplements (FS) are intended to complement and support basic nutritional needs. FS contain various nutritional components, among which plant extracts are common (1). These often include substances with clearly demonstrated clinical effects on health, as well as compounds with potential but not yet fully confirmed activity. The aim of this master’s thesis was to qualitatively characterize the presence of potentially bioactive compounds in maca-containing food supplements available on the Slovenian market and to assess the consistency between the declared claims and the actual chemical composition of the analysed products. Maca (Lepidium meyenii) is a traditional Peruvian plant, although it is now predominantly cultivated in China (3). We examined the Slovenian market and purchased ten FS containing maca as the main active ingredient. Based on a review of the scientific literature, we compiled a list of compounds believed to contribute to maca’s physiological effects and analysed the samples using UHPLC HRMS. We determined the presence of selected compounds in each product and, on this basis, assessed their potential activity. The data were processed using Xcalibur FreeStyle software. The analysis showed that the declared claims regarding the potential effects of the products generally correspond to the actual presence of compounds. Although our analysis included 101 potential compounds, approximately 20 components were detected across the samples. The detected compounds are consistent with effects attributed to maca in the literature and in product advertising, such as reduced fatigue, improved mood, and effects related to fertility. The results indicate that maca containing FS include compounds that may contribute to these effects; however, their presence alone does not confirm clinical efficacy but rather provides a biochemical basis for the advertised claims and for further research. Our study serves as a foundation for future quantitative analyses, in which the content of compounds should be determined using reference standards to evaluate whether the recommended daily doses of these FS are both safe and sufficient to achieve the intended effects. Based on the results of this master’s thesis, we conclude that the chemical composition of maca-containing food supplements varies among products, and that the detected compounds provide varying levels of support for the declared claims regarding their effects.

Keywords:Lepidium meyenii, UHPLC MS, qualitative analysis

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