Silica-coated SPIONs induce ferroptosis in endothelial cells while oleic acid mitigates their cytotoxic effects

Repar, Neža (author), Kupčič, Saša (author), Michelini, Sara (author), Jarc Jovičić, Eva (author), Kump, Ana (author), Hočevar, Matej (author), Kralj, Slavko (author), Petan, Toni (author), Drobne, Damjana (author)

Purpose: Induction of ferroptosis, a form of cell death driven by iron-dependent lipid peroxidation, holds promise as a novel cancer therapy. Superparamagnetic iron oxide nanoparticles (SPIONs) have been proven able to induce ferroptosis in tumour cells, while their effects on non-cancerous cells remain unclear. In this study, we investigated the ability of silica-coated SPIONs to induce ferroptosis in human umbilical vein endothelial cells (HUVEC) and explored the potential protective effects of oleic acid (OA). Additionally, we evaluated the applicability of scanning electron microscopy (SEM) in distinguishing between ferroptotic and apoptotic cell death. Results: We confirmed that silica-coated SPIONs, (used at concentrations of 25 and 50 μg/mL) increased lipid peroxidation and ROS formation in a dose-dependent manner up to 4.9- and 4-fold compared to controls, ultimately promoting ferroptosis without evidence of apoptosis, as indicated by the absence of phosphatidylserine-positive, propidium iodide-negative cells in flow cytometry experiments. Consistent with these results, the ferroptosis inhibitors α-tocopherol and ferrostatin-1 attenuated SPION-induced cytotoxicity, supporting ferroptosis as the primary mechanism of cell death. OA also protected cells from SPION-induced cytotoxicity by reducing lipid peroxidation, ROS formation, and cell death (from 58% to 26%), while increasing glutathione peroxidase expression. Unfortunately, due to the similar surface morphology of ferroptotic and apoptotic cells, SEM is not a reliable method for distinguishing between these two forms of cell death. Conclusion: This study provides important insights into the mechanisms of toxicity of silica-coated SPIONs in endothelial cells and highlights the potential role of OA as a modulator of SPION-induced side effects.

• Language: English
• Keywords: SPION, oleic acid, GPX4, ROS, ferroptosis, lipid peroxidation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: Str. 15441-15459
• Numbering: Vol. 20
• PID: 20.500.12556/RUL-182891
• UDC: 577
• ISSN on article: 1178-2013
• DOI: 10.2147/IJN.S548090
• COBISS.SI-ID: 264236291
• Publication date in RUL: 27.05.2026

Record is a part of a journal

• Title: International journal of nanomedicine
• Publisher: Dove Medical Press
• ISSN: 1178-2013
• COBISS.SI-ID: 3223828

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: superparamagnetni železovi oksidni nanodelci, oleinska kislina, glutation peroksidaza 4, reaktivne kisikove zvrsti, feroptoza, peroksidacija lipidov