Introduction: Assessment of knee joint laxity, an important aspect of joint stability, plays a role in diagnosis, rehabilitation monitoring, and evaluation of the risk of injury. Objective assessment of laxity using arthrometers provides more precise and valid measurements than manual tests, but their clinical applicability is limited by the lack of normative values, particularly for newer devices. Purpose: The aim of this study was to establish reference intervals for anterior and mediolateral rotational knee joint laxity, measured using the DYNEELAX® arthrometer, in healthy adult subjects, and to examine sex differences, lateral symmetry, differences between consecutive assessments, and the correlation between anterior and mediolateral rotational laxity. Methods: A cross-sectional study included 112 healthy adults aged 19–29 years. Laxity of a randomly selected knee joint was assessed twice consecutively in the sagittal and transverse planes. Anterior laxity was assessed at forces of 134 N, 150 N and 200 N, while mediolateral rotational laxity was assessed at torques of 3 N⋅m and 5 N⋅m. Data were analysed using standard statistical methods. Results: 50 % and 95 % reference intervals for anterior and mediolateral rotational knee joint laxity were determined in healthy adults aged 19–29 years. Women exhibited greater mediolateral rotational laxity compared to men, whereas differences in anterior laxity between sexes were not statistically significant. Anterior laxity values were lower in the right knee compared to the left, while differences in mediolateral rotational laxity between sides were not statistically significant. No statistically significant differences were observed between the first and second assessments. Anterior and mediolateral rotational laxity were negligibly to weakly correlated. Discussion and conclusion: For the first time, reference intervals for anterior and mediolateral rotational laxity were established in a large homogeneous sample. Compared to previous studies, the values were lower, likely due to higher stabilization forces and the measurement protocol used. In the sample, sex-related differences in mediolateral rotational laxity were clinically relevant, whereas differences between sides in anterior laxity were not clinically relevant. Future studies should investigate the influence of sex, side, and lower-limb dominance in larger samples, as well as determine reference intervals for posterior and valgus-varus laxity and for primary and secondary compliance.
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