Vpliv težkih kovin na rast studenčnega jetrenjaka (Marchantia polymorpha subsp. ruderalis) : magistrsko delo

Grandljič, Neja (author), Strgulc Krajšek, Simona (mentor)

V okviru magistrskega dela smo merili uspešnost rasti in vegetativnega razmnoževanja podvrste studenčnega jetrenjaka, Marchantia polymorpha subsp. ruderalis, ki je uspevala na zemlji z različno stopnjo onesnaženosti s težkimi kovinami. Preverili smo tudi, ali se pojavljajo razlike v rasti med rastlinami, ki so bile nabrane na različnih lokacijah po Sloveniji, ter uspešnost rasti moških in ženskih rastlin. Rastline smo nabrali v štirih mestih v Sloveniji, skupno na osmih nahajališčih in jih namnožili. V vsakem kraju smo nabrali vzorec moške in ženske rastline. Zemljo, onesnaženo s težkimi kovinami, smo nabrali na območju Žerjava, neonesnaženo zemljo pa na Jezerskem. Pripravili smo tudi mešanici teh dveh prsti. Rastline smo, izpostavljene različnim koncentracijam kovin, spremljali 35 dni. Ugotovili smo, da rastline najbolje rastejo na povsem neonesnaženi prsti. Rastline na onesnaženi prsti in v mešanici, ki je vsebovala večji delež žerjavske prsti, so rastle najslabše. Rastline, ki so rastle na mešanici onesnažene in neonesnažene prsti z manjšim deležem žerjavske prsti, pa so pokazale vmesno stopnjo rasti. Rastline, ki so rastle na neonesnaženi prsti, so razvile največ razplodnih košaric, najmanj pa so jih razvile rastline na onesnaženi prsti. Rastline, ki so rastle na mešanici onesnažene in neonesnažene prsti, pa so pokazale vmesno stopnjo razvoja razplodnih košaric. Opazili smo tudi znotrajvrstno variabilnost, saj so rastline, nabrane v Mariboru, rastle najbolje. Ugotovili smo, da ženske rastline na onesnaženih substratih bolje uspevajo od moških, ženske rastline pa so bolje od moških rastlin uspevale tudi glede na različen substrat. Rezultati kažejo, da je vrsta M. Polymorpha subsp. ruderalis občutljiva na povečano koncentracijo kovin v tleh.

• Language: Slovenian
• Keywords: biologija, rastline, studenčni jetrenjak, Marchantia polymorpha subsp. ruderalis, rast steljk, onesnaženost tal, težke kovine
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: PEF - Faculty of Education
• Place of publishing: Ljubljana
• Publisher: N. Grandljič
• Year: 2026
• Number of pages: X, 57 str.
• PID: 20.500.12556/RUL-181896
• UDC: 58(043.2)
• COBISS.SI-ID: 275771907
• Publication date in RUL: 18.04.2026

Secondary language

• Language: English
• Title: The effect of heavy metals on the growth of common liverwort (Marchantia polymorpha subsp. ruderalis)

Abstract

Growth and vegetative reproduction of the common liverwort, Marchantia polymorpha subsp. ruderalis, was assessed in soil with varying degrees of heavy metal contamination. We also examined whether there were differences in growth among plants collected from different locations in Slovenia and the growth success of male and female plants. Plants (both male and female) were collected from four cities in Slovenia, at a total of eight sites, and were multiplied from gemmae in the lab. Heavy metal-contaminated soil was collected from the Žerjav area, while uncontaminated soil was collected from Jezersko. Soil mixtures were also prepared to achieve a gradient in metal concentrations. Plants were grown in four treatments for 35 days. We found that plants grew best on uncontaminated soil. Plants on contaminated soil and in a mixture containing a higher proportion of Žerjav soil grew the worst. Plants that grew on a mixture of contaminated and uncontaminated soil with a smaller proportion of Žerjav soil showed an intermediate level of growth. Plants that grew on uncontaminated soil developed the largest number of gemmae cups, while plants on contaminated soil developed the fewest. Plants that grew on a mixture of contaminated and uncontaminated soil showed an intermediate level of gemmae cup development. We also observed intraspecific variability, as plants collected in Maribor grew best. Surprisingly, female plants performed better than male plants on contaminated substrates. Female plants also performed better than male plants across different substrates. Results indicate M. polymorpha subsp. ruderalis is sensitive to increasing concentrations of metals in the soil.

• Keywords: common liverwort, Marchantia polymorpha subsp. ruderalis, thallus growth, soil pollution, heavy metals