Effect of archaeal lipids from A. pernix K1 on the in vitro stability and active loading efficiency of liposomes composed of sphingomyelin and cholesterol

Kejžar, Jan (author), Skrt, Mihaela (author), Polajžer, Tamara (author), Poklar Ulrih, Nataša (author)

Archaeal lipids offer a useful strategy for improving liposomal systems because of their inherent stability under extreme conditions. incorporating lipids from Aeropyrum pernix K1 allowed us to optimize sphingomyelin-cholesterol formulation. these archaeal lipids enabled complete recovery of liposome morphology after destabilization with 4 mM ca2+ followed by eDta, indicating that membrane integrity is maintained in the presence of calcium. this is noteworthy given the anionic nature of the archaeal lipids, which in this case provide colloidal stability without increasing sensitivity to ca2+. the addition of 2 mol% mol archaeal lipids also supported high encapsulation efficiency of 95% for active vincristine loading and performed better than the binary mixture when vesicles were prepared by extrusion without cosolvents. Furthermore, reducing the archaeal lipid fraction preserved biological stability provided by sphingomyelin-cholesterol, as confirmed by flow cytometry and fluorescence microscopy, whereas pure archaeosomes composed entirely of archaeal lipids have been reported to show poor stability in biological systems.

• Language: English
• Keywords: archaea, lipids, liposomes, drug delivery systems, stability, vincristine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: In print
• Publication version: Version of Record
• Publication date: 01.01.2026
• Year: 2026
• Number of pages: Str. [v tisku, 1-15 str.]
• PID: 20.500.12556/RUL-181666
• UDC: 620.3:577.352:582.233
• ISSN on article: 0898-2104
• DOI: 10.1080/08982104.2026.2619597
• COBISS.SI-ID: 268189443
• Publication date in RUL: 10.04.2026

Record is a part of a journal

• Title: Journal of liposome research
• Publisher: Marcel Dekker
• ISSN: 0898-2104
• COBISS.SI-ID: 12563495

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: Aeropyrum pernix, arheje, biološke membrane, dostavni sistemi, fizikalno-kemijska stabilnost, fluidnost membran, fosfolipidi, holesterol, kalcein, kapsulacija, liposomi, sfingomielin, stabilnost in vitro, vinkristin

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0121
• Name: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi