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Successful purification of DNA from PFGE agarose plugs for whole genome sequencing
ID Strašek Smrdel, Katja (Author), ID Celar Šturm, Andraž (Author), ID Triglav, Tina (Author), ID Pirš, Mateja (Author)

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Abstract
Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

Language:English
Keywords:DNA purification, PFGE plugs, genotyping, bacterial isolates, WGS
Work type:Article
Typology:1.03 - Other scientific articles
Organization:BF - Biotechnical Faculty
MF - Faculty of Medicine
Publication status:Published
Publication version:Version of Record
Year:2025
Number of pages:Str. 1-5
Numbering:Vol. 68, no. 3
PID:20.500.12556/RUL-179378 This link opens in a new window
UDC:577
ISSN on article:1854-3073
DOI:10.14720/abs.68.3.21898 This link opens in a new window
COBISS.SI-ID:239702787 This link opens in a new window
Publication date in RUL:12.02.2026
Views:225
Downloads:92
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Record is a part of a journal

Title:Acta biologica slovenica
Publisher:Društvo biologov Slovenije, Društvo biologov Slovenije, Založba Univerze v Ljubljani, Društvo biologov Slovenije
ISSN:1854-3073
COBISS.SI-ID:221921024 This link opens in a new window

Secondary language

Language:Slovenian
Title:Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma
Abstract:
Sekvenciranje celotnega genoma (WGS) je nadomestilo tipizacijo bakterij na osnovi gelske pulzne elektroforeze (PFGE) kot referenčne metode genotipizacije. Raziskali smo primernost genomske DNA, očiščene iz agaroznih čepkov PFGE, shranjenih v laboratorijski zbirki, za WGS v primerih, ko bakterijski izolati niso več na voljo. Naša študija je pokazala, da je bakterijski WGS mogoče uspešno izvesti na DNA, očiščeni iz agaroznih čepkov PFGE.

Keywords:čiščenje DNA, čepki PFGE, genotipizacija, bakterijski izolati, WGS

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