Določitev vpliva citostatikov na kakovost plazmidne DNK pri kombinaciji elektrokemoterapije in genskega elektroprenosa

Novak, Ana (author), Kamenšek, Urška (mentor), Lampreht Tratar, Urša (comentor)

Rak je kompleksna bolezen, pri kateri ima imunski sistem ključno vlogo. Raziskave vse bolj usmerjajo terapije v aktivacijo protitumorskega imunskega odziva. Ena izmed obetavnih strategij je kombinacija elektrokemoterapije (EKT) in genskega elektroprenosa (GEP). Namen magistrskega dela je bil raziskati vpliv hkratne elektroporacije plazmidne DNK (pDNK) in citostatikov na učinkovitost transfekcije in citotoksičnost. Rezultati so pokazali, da citostatiki poškodujejo pDNK, vendar to ne zmanjša izražanja transgenov. Poleg tega hkratna elektroporacija ne zmanjša učinka EKT, temveč ga lahko poveča. Sklepamo, da je kombinirana uporaba EKT in GEP s hkratno elektroporacijo primerna in klinično obetavna terapija, ki bi lahko poenostavila protokole zdravljenja in izboljšala izid pri bolnikih.

• Language: Slovenian
• Keywords: onkologija, zdravljenje, imunoterapija, elektrokemoterapija, genski elektroprenos, cisplatin, bleomicin
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [A. Novak]
• Year: 2026
• PID: 20.500.12556/RUL-179325
• UDC: 616-006-085.84:602.6
• COBISS.SI-ID: 268515587
• Publication date in RUL: 11.02.2026

Secondary language

• Language: English
• Title: Determination of the effect of cytostatics on plasmid DNA quality in combination of electrochemotherapy and gene electrotransfer

Abstract

Cancer is a complex disease in which the immune system plays a key role. Research is increasingly focusing on therapies that activate antitumor immune responses. One of the promising strategies is the combination of electrochemotherapy (ECT) and gene electrotransfer (GET). The aim of this master’s thesis was to investigate the effects of simultaneous electroporation of plasmid DNA (pDNA) and cytostatic drugs on transfection efficiency and cytotoxicity. The results showed that cytostatic drugs damage pDNA; however, this does not reduce transgene expression. Furthermore, simultaneous electroporation does not diminish the effectiveness of ECT, but may even enhance it. We conclude that the combined use of ECT and GET with simultaneous electroporation is a suitable and clinically promising therapeutic approach that could simplify treatment protocols and improve patient outcomes.

• Keywords: oncology, treatment, immunotherapy, electrochemotherapy, gene electrotransfer, cisplatin, bleomycin