The effect of bisphenol PH and seven other bisphenol A alternatives on human haemostasis in vitro

Hrubša, Marcel (author), Soloviova, Alina (author), Harčárová, Patrícia (author), Gunaseelan, Catherine (author), Lomozová, Zuzana (author), Jirkovský, Eduard (author), Carazo, Alejandro (author), Sollner Dolenc, Marija (author), Peterlin-Mašič, Lucija (author), Mladěnka, Přemysl (author)

Recognition of bisphenol A’s toxicity has meant substitution by new analogues, which are poorly investigated. We determined the impact of bisphenol A and 8 of its analogues on platelet aggregation, coagulation, and their toxicity to erythrocytes. Erythrocyte lysis assays revealed bisphenol toxicity towards both human and rat erythrocytes with high TC$_{50s}$> 100 μM. Bisphenol A and bisphenol PH acted as antiplatelet compounds. Bisphenol PH was particularly potent (IC$_{50}$ of 0.42 ± 0.14 μM; 0.16 ± 0.05 μg/ml) in arachidonic acid-based platelet aggregation. Mechanistically, bisphenol PH blocked cyclooxygenase 1, in a similar manner to the antiplatelet drug acetylsalicylic acid. In terms of the coagulation cascade, only weak effects were found for some of the selected compounds, and the tested bisphenols did not impact coagulation or demonstrate erythrocytic toxicity at biologically achievable concentrations. Contrarily, the negative impact of bisphenol PH on platelets, with a possible subsequent risk of bleeding, might have biological relevance.

• Language: English
• Keywords: bisphenol, cytotoxicity, haemostasis, cyclooxygenase, platelet
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2026
• Number of pages: 1-9 str.
• Numbering: Vol. 121, [art. no.] ǂ104897
• PID: 20.500.12556/RUL-179102
• UDC: 615.9:612.43:620.266.1
• ISSN on article: 1872-7077
• DOI: 10.1016/j.etap.2025.104897
• COBISS.SI-ID: 260908547
• Publication date in RUL: 05.02.2026

Record is a part of a journal

• Title: Environmental toxicology and pharmacology
• Publisher: Elsevier
• ISSN: 1872-7077
• COBISS.SI-ID: 175280899

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: bisfenol, citotoksičnost, hemostaza, ciklooksigenaza, trombociti, toksikologija, endokrini vidik, hemostaza, strjevanje krvi, antikoagulacijsko zdravljenje, terapija z zdravili