Passage of the channel-forming agent nystatin through ergosterol-containing lipid membranes

Tinev, Megi (author), Kristanc, Luka (author), Gomišček, Gregor (author), Božič, Bojan (author)

The passage of nystatin through the ergosterol-containing phospholipid bilayer was studied on monovesicular and multivesicular giant unilamellar vesicles (GUVs and MVVs). Phase-contrast optical microscopy was used to examine vesicles composed of a palmitoyl-oleoyl-phosphatidyl-choline (POPC) bilayer with either 15 or 45 mol% ergosterol. Three types of vesicles were analyzed: (i) GUVs, (ii) outer vesicles (outGUVs) of MVVs, and (iii) inner vesicles (inGUVs) of MVVs. The times of their ruptures were determined after their exposure to nystatin at concentrations of 250 and 500 μ M. At both concentrations, the times that the inGUVs spent in the nystatin solution after the rupture of the corresponding outGUVs were significantly shorter than the rupture times of individual GUVs of the similar size. These differences in rupture times demonstrate that the ergosterol-containing POPC membrane is permeable to nystatin.

• Language: English
• Keywords: phospholipid vesicle, ergosterol, nystatin, transmembrane passage
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2025
• Year: 2025
• Number of pages: Str. 415-427
• Numbering: Vol. 258, no. 5
• PID: 20.500.12556/RUL-178195
• UDC: 577
• ISSN on article: 1432-1424
• DOI: 10.1007/s00232-025-00354-3
• COBISS.SI-ID: 243700995
• Publication date in RUL: 21.01.2026

Record is a part of a journal

• Title: The journal of membrane biology
• Shortened title: J. membr. biol.
• Publisher: Springer
• ISSN: 1432-1424
• COBISS.SI-ID: 512024345

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: fosfolipidni vezikel, ergosterol, nistatin, transmembranski prehod

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P1-0055-2022
• Name: Biofizika polimerov, membran, gelov, koloidov in celic