First case report of Shewanella indica isolated from a hospitalized patient in Serbia

Filipić, Brankica (author), Žugić, Vladimir (author), Golob, Majda (author), Hrast Rambaher, Martina (author), Bošković, Lidija (author), Milenković, Marina T. (author)

Over the past decade, Shewanella spp. have been increasingly recognized as opportunistic pathogens, particularly in patients with malignancies, neutropenia, severe heart failure, renal insufficiency, and hepatobiliary diseases. Shewanella indica is a rarely reported species within the Shewanella genus, and its role in human infection remains poorly documented. In this study, we report the first case of S. indica isolated from the stool of a critically ill 72-year-old man in Serbia with multiple pre-existing comorbidities and co-infections. Initial identification using the VITEK 2 system misclassified the strain as Shewanella algae, while species-level identification by the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was inconclusive. However, whole-genome sequencing (WGS) definitively identified the isolate as S. indica and revealed a multidrug-resistant profile together with numerous virulence-associated genes. Minimum inhibitory concentrations (MICs) were determined for 32 antibiotics, although interpretation was constrained by the lack of species-specific breakpoints. This case highlights the diagnostic challenges in differentiating Shewanella indica, discusses its possible clinical roles, and underscores the value of genomic tools for accurate identification. It also reinforces the importance of recognizing rare pathogens in complex clinical scenarios.

• Language: English
• Keywords: Shewanella indica, Zn4(μ4-O) entity, genome sequencing, species Identification, and antimicrobial susceptibility
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty; FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2026
• Number of pages: 8 str.
• Numbering: Vol. 12, art. 1715579
• PID: 20.500.12556/RUL-178002
• UDC: 579:616
• ISSN on article: 2296-858X
• DOI: 10.3389/fmed.2025.1715579
• COBISS.SI-ID: 264321539
• Publication date in RUL: 16.01.2026

Record is a part of a journal

• Title: Frontiers in medicine
• Shortened title: Front. med.
• Publisher: Frontiers Media
• ISSN: 2296-858X
• COBISS.SI-ID: 523095065

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: Shewanella indica, klinični primer, sekvenciranje genoma, identifikacija do vrste in odpornost proti antibiotikom