Introduction: Muscle injuries among football players account for 30% of all injuries and cause approximately a quarter of player absences. The hamstrings are the most commonly injured muscles, with the biceps femoris being the most commonly affected. Tensiomyography has been widely used to determine muscle properties. Purpose: The purpose of the master's thesis is to determine whether tensiomyography and the measurements performed with it can predict hamstring injury to football players of the First Slovenian Football League. Methods: The study included football players who played in the First Slovenian Football League in the 2018/2019, 2019/2020, 2022/2023 and 2023/2024 seasons. Using tensiomyography, we measured the contractile properties of the rectus femoris, vastus medialis, vastus lateralis, biceps femoris and semitendinosus muscles for both lower limbs before each season. Football clubs reported hamstring injuries that occurred after the measurements were taken in each season. The Kolmogorov-Smirnov test was used to check the normal distribution of the data. Differences in selected variables measured by tensiomyography were tested for normally distributed data with the independent samples t-test, and for non-normally distributed data with the Mann-Whitney U-test. Binary logistic regression was used to predict hamstring injuries. Statistical significance was obtained at p < 5%. Results: The study included 365 football players. After the measurements were taken, 19 suffered a hamstring injury. A statistically significant difference (U = 21582.0, p = 0.011) between the two groups was detected in the age of the participants, with injured players being significantly older than uninjured players. A statistically significant difference was found for the Td parameter of the biceps femoris muscle of the left leg (U = 2347, p = 0.036) between the injured and uninjured football players. In the binary logistic regression analysis, the Td parameter of the biceps femoris muscle of the left leg proved to be a statistically significant predictor of hamstring injury (p = 0.015). Discussion and conclusion: The Td parameter for the biceps femoris muscle on the left leg (Bf_Td_L ) proved to be a statistically significant predictor of hamstring injury in football players, but additional analysis did not detect a statistically significant difference for the parameter Bf_Td_L and hamstring injury on the left or right leg and on the dominant or non-dominant leg, so it seems most likely that the association between the parameter Bf_Td_L and hamstring injury is coincidental due to the small number of injured football players.
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