Introduction: Hand disinfection is one of the most important hygiene measures in preventing the transmission of hospital-acquired infections. Despite numerous awareness campaigns and the development of international and national guidelines, the number of healthcare-associated infections continues to rise. Therefore, further awareness-raising and harmonization of all public, international, and internal recommendations are necessary. Purpose: The purpose of this thesis is to present the importance of hand disinfection among healthcare workers as a fundamental measure in preventing the spread of microorganisms and to compare international and internal hand hygiene guidelines used in Slovenian hospitals. Methods: A descriptive research method was used with a narrative review of professional and scientific literature published between 2015 and 2025 in the databases CINAHL Ultimate, MEDLINE/PubMed, ScienceDirect, and the Digital Library of the University of Ljubljana. Internal guidelines from five Slovenian hospitals were analysed and compared with the World Health Organization, Centers for Disease Control and Prevention, Kommission für Krankenhaushygiene und Infektionsprävention, and Association of periOperative Registered Nurses recommendations. Results: The analysis showed that international and internal guidelines are based on similar principles but differ in the duration of hand rubbing, the amount of disinfectant used, and whether the wrists are included in the procedure. Differences were also found regarding nail length, the use of nail polish, and hand care. Discussion and conclusion: Compliance with hand hygiene practices is influenced by various factors, including lack of time, workload, and inconsistent instructions. Differences between guidelines may affect the quality of hand disinfection; therefore, continuous education and regular monitoring of proper hand hygiene practices are essential. Hand disinfection remains a key measure in preventing hospital-acquired infections. Further research comparing the effectiveness of different approaches—regarding rubbing time, disinfectant volume, and wrist inclusion—would help to standardize guidelines and improve patient safety.
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