Navidezna prebavljivost surovih beljakovin in surovih maščob pri linijah miši selekcioniranih na višji oziroma nižji odstotek telesnih maščob

Trebušak, Tina (author), Pirman, Tatjana (author), Horvat, Simon (author)

Debelost in naraščanje indeksa telesne mase (ITM) predstavljata v današnjem času velik zdravstveni problem pri ljudeh, prekomerna zamaščenost pa je prav tako nezaželena pri reji domačih živali, saj poslabšuje gospodarnost prireje. V naši raziskavi smo preučevali vpliv debelosti laboratorijskih miši na navidezno prebavljivost surovih beljakovin in surovih maščob zaužite krme. V dveh prebavljivostnih poskusih smo uporabili dve selekcijski liniji miši, linijo F (višji odstotek telesnega maščevja) in L (nižji odstotek telesnega maščevja). V prvem poskusu smo uporabili 21 samcev obeh linij (11 linije F in 10 linije L), starih od 9.0 do 18,6 tednov, v drugem pa 23 samcev (11 linije F in 12 linije L) starih od 9,6 do 11,00 tednov. V času poskusa (5 zaporednih dni) smo živali dnevno tehtali, merili količino zaužite krme in zbirali blato. Po postopkih weendske analize smo v vzorcih določili vsebnost surovih beljakovin in surovih maščob v krmi in blatu. Rezultati so pokazali, da med linijama F in L obstajajo statistično značilne razlike v navidezni prebavljivosti surovih beljakovin in surovih maščob. Pri zauživanju krme ni bilo statistično značilnih razlik med linijama, so se pa pokazale statistično značilne razlike pri zaužiti krmi na g telesne mase. Linija L je bolje prebavila tako surove beljakovine (79,6%) kot tudi surove maščobe (91,8%), v primerjavi z linijo F, pri kateri je bila navidezna prebavljivost v povprečju 77,1% oziroma 87,0% za surove beljakovine in surove maščobe. Glede na dobljene rezultate sklepamo, da z razlikami v navidezni prebavljivosti beljakovin in maščob krme ne moremo razložiti velikih fenotipskih razlik med linijama.

• Language: Slovenian
• Keywords: domače živali, prehrana živali, prehrana ljudi, zamaščenost, surove beljakovine, surove maščobe, prebavljivost, selekcija, maščevje, linije, laboratorijske miši
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2009
• Number of pages: Str. 33-38
• Numbering: Letn. 94, št. 1
• PID: 20.500.12556/RUL-17701
• UDC: 636.084/.087:613.2
• ISSN on article: 1581-9175
• COBISS.SI-ID: 2539656
• Publication date in RUL: 11.07.2014

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Shortened title: Acta agric. Slov.
• Publisher: Biotehniška fakulteta
• ISSN: 1581-9175
• COBISS.SI-ID: 213840640

Secondary language

• Language: English

Abstract

Obesity and increasing of body mass index (BMI) present a growing health problem and are also an unwanted component of growth in domestic animals. The main objective of this study was to examine the effect of obesity on apparent digestibility of diet crude protein and fat in mice. In two susequent experiments digestibility was compared between two selection mouse lines, F line (selected for higher percentage of body fat) and L (selected for lower percentage of body fat). In the first experiment 21 males of both lines (11 F and 10L) between 9.0 and 18.6 weeks of age were used whereas the second experiment involved 23 males (11 F and 12 L) between 9.6 and 11.0 weeks of age. Weights of mice, faeces and food intake were recorded daily for 5 successive days. Using Weende analysis the content of crude protein and crude fat in diet and faeces were determined. The results of our study revealed statistically significant differences in the apparent digestibility of crude protein and crude fat between the F and L line. There were also significant differences in diet intake per g of body weight, but not in the total amount of all consumed diet between the lines. The crude protein and the crude fat were digested more efficiency by the L line (79.6% and 91.8%, respectively) while the F line digested 77.1% of crude protein and 87.0% of crude fat. Considering these results the differernces in the apparent digestibility of crude protein and crude fat can not explain great differences in obesity between the F and L line.

• Keywords: farm animals, animal nutrition, obesity, crude protein, crude fat, digestibility, selection, body fat, lines, laboratory mice